array:23 [
  "pii" => "S1579212907601256"
  "issn" => "15792129"
  "doi" => "10.1016/S1579-2129(07)60125-6"
  "estado" => "S300"
  "fechaPublicacion" => "2007-01-01"
  "aid" => "60125"
  "copyright" => "Sociedad Española de Neumología y Cirugía Torácica (SEPAR)"
  "copyrightAnyo" => "2007"
  "documento" => "article"
  "crossmark" => 0
  "subdocumento" => "fla"
  "cita" => "Arch Bronconeumol. 2007;43:542-8"
  "abierto" => array:3 [
    "ES" => false
    "ES2" => false
    "LATM" => false
  ]
  "gratuito" => false
  "lecturas" => array:2 [
    "total" => 2064
    "formatos" => array:3 [
      "EPUB" => 79
      "HTML" => 1336
      "PDF" => 649
    ]
  ]
  "itemSiguiente" => array:18 [
    "pii" => "S1579212907601268"
    "issn" => "15792129"
    "doi" => "10.1016/S1579-2129(07)60126-8"
    "estado" => "S300"
    "fechaPublicacion" => "2007-01-01"
    "aid" => "60126"
    "copyright" => "Sociedad Española de Neumología y Cirugía Torácica (SEPAR)"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Arch Bronconeumol. 2007;43:549-56"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 2389
      "formatos" => array:3 [
        "EPUB" => 88
        "HTML" => 1689
        "PDF" => 612
      ]
    ]
    "en" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
      "titulo" => "Trends in Prognostic Factors for Neuroendocrine Lung Tumors"
      "tienePdf" => "en"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "549"
          "paginaFinal" => "556"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Mariano Garc&#237;a-Yuste, Laureano Molins, Jos&#233; M&#46; Matilla, Federico Gonz&#225;lez-Aragoneses, Javier L&#243;pez-Pujol, Guillermo Ramos, Mercedes de la Torre"
          "autores" => array:8 [
            0 => array:2 [
              "nombre" => "Mariano"
              "apellidos" => "Garc&#237;a-Yuste"
            ]
            1 => array:2 [
              "nombre" => "Laureano"
              "apellidos" => "Molins"
            ]
            2 => array:2 [
              "nombre" => "Jos&#233; M&#46;"
              "apellidos" => "Matilla"
            ]
            3 => array:2 [
              "nombre" => "Federico"
              "apellidos" => "Gonz&#225;lez-Aragoneses"
            ]
            4 => array:2 [
              "nombre" => "Javier"
              "apellidos" => "L&#243;pez-Pujol"
            ]
            5 => array:2 [
              "nombre" => "Guillermo"
              "apellidos" => "Ramos"
            ]
            6 => array:2 [
              "nombre" => "Mercedes"
              "apellidos" => "de la Torre"
            ]
            7 => array:1 [
              "colaborador" => "members of the Spanish Multicenter Study of Neuroendocrine Lung Tumors of the Spanish Society of Pulmonology and Thoracic Surgery &#40;EMETNE-SEPAR&#41;"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907601268?idApp=UINPBA00003Z"
    "url" => "/15792129/0000004300000010/v1_201305150259/S1579212907601268/v1_201305150259/en/main.assets"
  ]
  "itemAnterior" => array:18 [
    "pii" => "S1579212907601244"
    "issn" => "15792129"
    "doi" => "10.1016/S1579-2129(07)60124-4"
    "estado" => "S300"
    "fechaPublicacion" => "2007-01-01"
    "aid" => "60124"
    "copyright" => "Sociedad Espa&#241;ola de Neumolog&#237;a y Cirug&#237;a Tor&#225;cica &#40;SEPAR&#41;"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Arch Bronconeumol. 2007;43:535-41"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 4126
      "formatos" => array:3 [
        "EPUB" => 86
        "HTML" => 3263
        "PDF" => 777
      ]
    ]
    "en" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
      "titulo" => "Variability in Peak Expiratory Flow Does Not Classify Asthma According to Severity"
      "tienePdf" => "en"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "535"
          "paginaFinal" => "541"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Eduardo G&#46; P&#233;rez-Yarza, Nicol&#225;s Cobos, Juan Jos&#233; de la Cruz"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "Eduardo G&#46;"
              "apellidos" => "P&#233;rez-Yarza"
            ]
            1 => array:2 [
              "nombre" => "Nicol&#225;s"
              "apellidos" => "Cobos"
            ]
            2 => array:2 [
              "nombre" => "Juan Jos&#233;"
              "apellidos" => "de la Cruz"
            ]
            3 => array:1 [
              "colaborador" => "on behalf of the Asthma Working Group of the Spanish Society of Pediatric Pneumology"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907601244?idApp=UINPBA00003Z"
    "url" => "/15792129/0000004300000010/v1_201305150259/S1579212907601244/v1_201305150259/en/main.assets"
  ]
  "en" => array:15 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
    "titulo" => "Interleukin-8 Expression in Lung Tissue During Ischemia-Reperfusion"
    "tieneTextoCompleto" => 0
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "542"
        "paginaFinal" => "548"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "Jos&#233; Mar&#237;a Matilla, Mariano Garc&#237;a-Yuste, Mariano S&#225;nchez Crespo, Manuel J&#46; Gayoso, F&#233;lix Heras, Marta Jim&#233;nez Prada, Andr&#233;s Alonso, Guillermo Ramos"
        "autores" => array:8 [
          0 => array:4 [
            "nombre" => "Jos&#233; Mar&#237;a"
            "apellidos" => "Matilla"
            "email" => array:1 [
              0 => "jmmatilla17&#64;hotmail&#46;com"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor1"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "Mariano"
            "apellidos" => "Garc&#237;a-Yuste"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "Mariano S&#225;nchez"
            "apellidos" => "Crespo"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          3 => array:2 [
            "nombre" => "Manuel J&#46;"
            "apellidos" => "Gayoso"
          ]
          4 => array:3 [
            "nombre" => "F&#233;lix"
            "apellidos" => "Heras"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          5 => array:3 [
            "nombre" => "Marta Jim&#233;nez"
            "apellidos" => "Prada"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          6 => array:3 [
            "nombre" => "Andr&#233;s"
            "apellidos" => "Alonso"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          7 => array:3 [
            "nombre" => "Guillermo"
            "apellidos" => "Ramos"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:2 [
          0 => array:3 [
            "entidad" => "Unidad de Cirug&#237;a Tor&#225;cica Experimental&#44; Facultad de Medicina&#44; Universidad de Valladolid&#44; Valladolid&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "aff1"
          ]
          1 => array:3 [
            "entidad" => "Instituto de Biolog&#237;a y Gen&#233;tica Molecular &#40;IBGM&#41;&#44; Universidad de Valladolid-CSIC&#44; Valladolid&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "aff2"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor1"
            "etiqueta" => "&#42;"
            "correspondencia" => "Correspondence&#58; Dr&#46; J&#46;M&#46; Matilla Gonz&#225;lez&#46; Servicio de Cirug&#237;a Tor&#225;cica&#46; Hospital Cl&#237;nico Universitario de Valladolid&#46; Ram&#243;n y Cajal&#44; 3&#46; 47005 Valladolid&#46; Espa&#241;a"
          ]
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2004-08-16"
    "fechaAceptado" => "2006-10-24"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Key words"
          "identificador" => "xpalclavsec154621"
          "palabras" => array:4 [
            0 => "Ischemia-reperfusion"
            1 => "Interleukin 8"
            2 => "Polymorphonuclear leukocytes"
            3 => "Lung transplantation"
          ]
        ]
      ]
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec154622"
          "palabras" => array:4 [
            0 => "Isquemia-reperfusi&#243;n"
            1 => "Interleucina-8"
            2 => "Leucocitos polimorfonucleares"
            3 => "Trasplante pulmonar"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:1 [
        "resumen" => "<span class="elsevierStyleSectionTitle">Objective</span><p class="elsevierStyleSimplePara elsevierViewall">Local cytokine production is a pathogenic factor in ischemia-reperfusion injury in early graft dysfunction&#46; This study analyzed interleukin 8 &#40;IL-8&#41; messenger RNA &#40;mRNA&#41; expression in lung tissue and the association between IL-8 mRNA levels and interstitial lung changes in an experimental model of warm lung ischemia-reperfusion&#46;</p> <span class="elsevierStyleSectionTitle">MATERIAL AND METHODS</span><p class="elsevierStyleSimplePara elsevierViewall">We studied 16 New Zealand rabbits divided into 3 groups&#58; control&#44; ischemia &#40;tissue taken from right lower lobe after 1&#44; 2&#44; or 3 hours of ischemia&#41;&#44; and reperfusion &#40;tissue taken from right upper and middle lobes after 1 hour of ischemia and 1&#44; 2&#44; or 3 hours of reperfusion&#41;&#46; Expression of IL-8 mRNA was determined by reverse transcription and polymerase chain reaction&#46; Interstitial infiltration by polymorphonuclear neutrophils was determined&#46; The Mann-Whitney U-test was used for statistical comparisons&#44; with <span class="elsevierStyleItalic">P</span>&#60;&#46;05 considered to indicate a significant result&#46;</p> <span class="elsevierStyleSectionTitle">RESULTS</span><p class="elsevierStyleSimplePara elsevierViewall">During ischemia&#44; IL-8 mRNA levels were elevated at the end of hour 1 &#40;<span class="elsevierStyleItalic">P</span>&#61;&#46;009&#41; with respect to the control group&#44; but not thereafter&#46; Interstitial changes were minimal&#46; IL-8 mRNA levels during reperfusion were similar to those observed during ischemia&#44; with a slight increase at the end of hour 2&#46; There were no significant differences between hours 1&#44; 2&#44; and 3&#46; Polymorphonuclear neutrophil recruitment occurred at the beginning of reperfusion &#40;<span class="elsevierStyleItalic">P</span>&#61;&#46;014&#41;&#44; but no significant differences were observed at hours 2 or 3&#46; Progressive thickening of alveolar septa and edema was documented&#46;</p> <span class="elsevierStyleSectionTitle">CONCLUSIONS</span><p class="elsevierStyleSimplePara elsevierViewall">Changes in IL-8 mRNA expression during ischemia precede interstitial infiltration by polymorphonuclear neutrophils during reperfusion&#44; suggesting that the 2 processes are related&#46; Quantification of IL-8 mRNA expression could facilitate early diagnosis of graft dysfunction&#46;</p>"
      ]
      "es" => array:1 [
        "resumen" => "<span class="elsevierStyleSectionTitle">Objetivo</span><p class="elsevierStyleSimplePara elsevierViewall">La generaci&#243;n local de citocinas es un factor patog&#233;nico en el da&#241;o por isquemia-reperfusi&#243;n en la disfunci&#243;n precoz del injerto&#46; Este estudio analiza la expresi&#243;n en tejido pulmonar de ARN mensajero de interleucina-8 &#40;ARNm de IL-8&#41; y su relaci&#243;n con los cambios intersticiales pulmonares en un modelo experimental de isquemia-reperfusi&#243;n pulmonar normot&#233;rmica&#46;</p> <span class="elsevierStyleSectionTitle">MATERIAL Y M&#201;TODOS</span><p class="elsevierStyleSimplePara elsevierViewall">Se estudiaron 16 conejos de la raza Nueva Zelanda en 3 grupos de estudio&#58; a&#41; basal&#59; b&#41; isquemia &#40;l&#243;bulo inferior derecho tras isquemia de 1&#44; 2 o 3 h&#41;&#44; y c&#41; reperfusi&#243;n &#40;l&#243;bulos superior y medio derechos tras 1 h de isquemia y 1&#44; 2 o 3 h de reperfusi&#243;n&#41;&#46; Se deter- min&#243; la expresi&#243;n del ARNm de IL-8 mediante transcripci&#243;n inversa y reacci&#243;n en cadena de la polimerasa y estudi&#243; la infiltraci&#243;n intersticial por polimorfonucleares &#40;PMN&#41;&#46; Para el an&#225;lisis estad&#237;stico se emple&#243; el test de la U de Mann-Whitney aceptando como significativo un valor de p &#60; 0&#44;05&#46;</p> <span class="elsevierStyleSectionTitle">RESULTADOS</span><p class="elsevierStyleSimplePara elsevierViewall">Durante el per&#237;odo de isquemia se observ&#243; respecto al basal elevaci&#243;n del ARNm de IL-8 al final de la primera hora &#40;p &#61; 0&#44;009&#41;&#44; pero no durante el resto del per&#237;odo isqu&#233;mico&#46; Los cambios intersticiales fueron m&#237;nimos&#46; Durante la reperfusi&#243;n los valores de ARNm de IL-8 fueron semejantes a los observados durante la isquemia&#44; con una ligera elevaci&#243;n al final de segunda hora&#59; no hubo diferencias significativas entre la primera&#44; segunda y tercera horas&#46; Hubo reclutamiento de PMN al inicio de la reperfusi&#243;n &#40;p &#61; 0&#44;014&#41;&#44; sin observarse diferencias significativas en la segunda y tercera horas&#46; Se objetiv&#243; un engrosamiento progresivo de los tabiques interalveolares y edema&#46;</p> <span class="elsevierStyleSectionTitle">CONCLUSIONES</span><p class="elsevierStyleSimplePara elsevierViewall">Los cambios en la expresi&#243;n del ARNm de IL-8 durante la isquemia preceden a la infiltraci&#243;n intersticial de PMN durante la reperfusi&#243;n&#44; lo que se&#241;ala una relaci&#243;n entre ambos procesos&#46; La cuantificaci&#243;n del ARNm de IL-8 podr&#237;a ser un procedimiento para el seguimiento diagn&#243;stico de la disfunci&#243;n precoz del injerto&#46;</p>"
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:1 [
        "nota" => "<p class="elsevierStyleNotepara">This study was realized with the scientific help and the financiation of the Sociedad Espa&#241;ola de Neumolog&#237;a y Cirug&#237;a Tor&#225;cica&#46; &#40;SEPAR&#41;&#46;</p>"
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "REFERENCES"
      "seccion" => array:1 [
        0 => array:1 [
          "bibliografiaReferencia" => array:22 [
            0 => array:3 [
              "identificador" => "bib1"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Early release of proinflammatory cytokines after lung transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "H Mal"
                            1 => "M Dehoux"
                            2 => "C Sleiman"
                            3 => "J Boczkowski"
                            4 => "G Les&#233;che"
                            5 => "R Pariente"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "1998"
                        "volumen" => "113"
                        "paginaInicial" => "645"
                        "paginaFinal" => "651"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9515837"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib2"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Potential role of monocyte chemoattractant protein 1&#47;JE in monocyte&#47; macrophage-dependent IgA immune complex alveolitis in the rat"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "mL Jones"
                            1 => "MS Mulligan"
                            2 => "CM Flory"
                            3 => "PA Ward"
                            4 => "JS Warren"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Immunol"
                        "fecha" => "1992"
                        "volumen" => "149"
                        "paginaInicial" => "2147"
                        "paginaFinal" => "2154"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1387671"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib3"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Requirement for C-X-C chemokines &#40;macrophage inflammatory protein-2&#44; and cytokine-induced neutrophil chemoattractant&#41; in IgG immune complex-induced lung injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "TP Shanley"
                            1 => "H Schmal"
                            2 => "RL Warner"
                            3 => "E Schmid"
                            4 => "HP Friedl"
                            5 => "PA Ward"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "J Immunol"
                        "fecha" => "1997"
                        "volumen" => "158"
                        "paginaInicial" => "3439"
                        "paginaFinal" => "3448"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9120305"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S0002937808002949"
                          "estado" => "S300"
                          "issn" => "00029378"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib4"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Regulation of chemokine expression by cyclosporine A in alveolar macrophages exposed to hypoxia and reoxygenation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "BV Naidu"
                            1 => "B Krishnadasan"
                            2 => "K Byrne"
                            3 => "AL Farr"
                            4 => "M Rosengart"
                            5 => "ED Verrier"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "2002"
                        "volumen" => "74"
                        "paginaInicial" => "899"
                        "paginaFinal" => "905"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12238858"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S0735109701014279"
                          "estado" => "S300"
                          "issn" => "07351097"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib5"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung transplantation&#58; a decade of experience"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "BF Meyer"
                            1 => "J Lynch"
                            2 => "EP Trulock"
                            3 => "TJ Guthrie"
                            4 => "JD Cooper"
                            5 => "GA Patterson"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Surg"
                        "fecha" => "1999"
                        "volumen" => "230"
                        "paginaInicial" => "362"
                        "paginaFinal" => "367"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10493483"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib6"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Purification and partial biologic characterization of a human lymphocyte derived peptide with potent neutrophil stimulating activity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "JM Schr&#246;der"
                            1 => "U Mrowietz"
                            2 => "E Christophers"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "J Immunol"
                        "fecha" => "1988"
                        "volumen" => "140"
                        "paginaInicial" => "3534"
                        "paginaFinal" => "3540"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3283239"
                            "web" => "Medline"
                          ]
                        ]
                        "itemHostRev" => array:3 [
                          "pii" => "S073510979900412X"
                          "estado" => "S300"
                          "issn" => "07351097"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib7"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Anoxia-hyperoxia induces monocyte derived interleukin-8"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "AP Metinko"
                            1 => "SL Kunkel"
                            2 => "TJ Standiford"
                            3 => "RM Strieter"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1172/JCI115953"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Invest"
                        "fecha" => "1992"
                        "volumen" => "90"
                        "paginaInicial" => "791"
                        "paginaFinal" => "798"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1522234"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib8"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "EPC-K1 is effective in lung preservation in a ex vivo rabbit lung perfusion model"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "I Nagahiro"
                            1 => "M Aoe"
                            2 => "M Yamashita"
                            3 => "H Date"
                            4 => "A Andou"
                            5 => "N Shimizu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1997"
                        "volumen" => "63"
                        "paginaInicial" => "954"
                        "paginaFinal" => "959"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9124970"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib9"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Changes in vascular permeability with ischemic time&#44; temperature&#44; and inspired oxygen fraction in isolated rabbit lungs"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "M Haniuda"
                            1 => "CM Dresler"
                            2 => "S Hasegawa"
                            3 => "GA Patterson"
                            4 => "JD Cooper"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1994"
                        "volumen" => "57"
                        "paginaInicial" => "708"
                        "paginaFinal" => "714"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7511884"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib10"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effects of inflammation volume during lung preservation on pulmonary capillary permeability"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "M Haniuda"
                            1 => "S Hasegawa"
                            2 => "T Shiraishi"
                            3 => "CM Dresler"
                            4 => "JD Cooper"
                            5 => "GA Patterson"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Thorac Cardiovasc Surg"
                        "fecha" => "1996"
                        "volumen" => "112"
                        "paginaInicial" => "85"
                        "paginaFinal" => "93"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8691890"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib11"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Purification and partial biologic characterization of a human lymphocyte derived peptide with potent neutrophil stimulating activity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "JM Schr&#246;der"
                            1 => "U Mrowietz"
                            2 => "E Christophers"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Immunol"
                        "fecha" => "1988"
                        "volumen" => "140"
                        "paginaInicial" => "3534"
                        "paginaFinal" => "3540"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3283239"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib12"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Bactericidal&#47;permeability-increasing protein attenuates systemic inflammation and acute lung injury in porcine lower limb ischemia-reperfusion injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "DW Harkin"
                            1 => "AB Barros"
                            2 => "K McCallion"
                            3 => "M Hoper"
                            4 => "MI Halliday"
                            5 => "FC Campbell"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Surg"
                        "fecha" => "2001"
                        "volumen" => "234"
                        "paginaInicial" => "233"
                        "paginaFinal" => "244"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11505070"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib13"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The release of cytokines&#44; adhesion molecules and extracellular matrix parameters during and after reperfusion in human liver transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "AR Mueller"
                            1 => "KP Platz"
                            2 => "M Haak"
                            3 => "H Undi"
                            4 => "C Muller"
                            5 => "E Kottgen"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Transplantation"
                        "fecha" => "1996"
                        "volumen" => "62"
                        "paginaInicial" => "1118"
                        "paginaFinal" => "1126"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8900313"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib14"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "N Sekido"
                            1 => "N Mukaida"
                            2 => "A Harada"
                            3 => "I Nakanishi"
                            4 => "Y Watanabe"
                            5 => "K Matsushima"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1038/365654a0"
                      "Revista" => array:6 [
                        "tituloSerie" => "Nature"
                        "fecha" => "1993"
                        "volumen" => "365"
                        "paginaInicial" => "654"
                        "paginaFinal" => "657"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8413628"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib15"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ischemia-reperfusion lung injury in rabbits&#58; mechanisms of injury and protection"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "T Sakuma"
                            1 => "K Takahashi"
                            2 => "N Ohya"
                            3 => "O Kajikawa"
                            4 => "TR Martin"
                            5 => "AMA Matthay"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Am J Physiol Lung Cell Mol Physiol"
                        "fecha" => "1999"
                        "volumen" => "276"
                        "paginaInicial" => "L137"
                        "paginaFinal" => "LL45"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib16"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Interleukin-8 release during early reperfusion predicts graft function in human lung transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "M de Perrot"
                            1 => "Y Sekine"
                            2 => "S Fischer"
                            3 => "TK Waddell"
                            4 => "K McRae"
                            5 => "M Liu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.165.2.2011151"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2002"
                        "volumen" => "165"
                        "paginaInicial" => "211"
                        "paginaFinal" => "215"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11790657"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib17"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Elevated levels of interleukin-8 in donor lungs is associated with early graft failure after lung transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "AJ Fisher"
                            1 => "SC Donnelly"
                            2 => "N Hirani"
                            3 => "C Haslett"
                            4 => "RM Strieter"
                            5 => "JH Dark"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2001"
                        "volumen" => "63"
                        "paginaInicial" => "259"
                        "paginaFinal" => "265"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib18"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Microvascular permeability of the non-heart beating rabbit lung after warm ischemia and reperfusion&#58; role of neutrophil elastase"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "H Kishima"
                            1 => "SI Takeda"
                            2 => "S Miyoshi"
                            3 => "A Matsumura"
                            4 => "M Minami"
                            5 => "T Utsumi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1998"
                        "volumen" => "65"
                        "paginaInicial" => "913"
                        "paginaFinal" => "918"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9564900"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib19"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Early cellular events in the lung allograft"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "R Adoumie"
                            1 => "C Serrick"
                            2 => "A Giaid"
                            3 => "H Shennib"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1992"
                        "volumen" => "54"
                        "paginaInicial" => "1071"
                        "paginaFinal" => "1077"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1449289"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib20"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Neutrophils are not necessary for ischemia-reperfusion lung injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "CN Steimle"
                            1 => "TD Guynn"
                            2 => "mL Morganroth"
                            3 => "SF Bolling"
                            4 => "K Carr"
                            5 => "M Deeb"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1992"
                        "volumen" => "53"
                        "paginaInicial" => "64"
                        "paginaFinal" => "73"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1728243"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib21"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ischemia-reperfusion induced lung injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "M de Perrot"
                            1 => "M Liu"
                            2 => "TK Waddell"
                            3 => "S Keshavjee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.200207-670SO"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2003"
                        "volumen" => "167"
                        "paginaInicial" => "490"
                        "paginaFinal" => "511"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12588712"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib22"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Controlled reperfusion prevents pulmonary injury after 24 hours of lung preservation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "AO Halldorsson"
                            1 => "M Kronon"
                            2 => "BS Allen"
                            3 => "S Rahman"
                            4 => "T Wang"
                            5 => "M Layland"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1998"
                        "volumen" => "66"
                        "paginaInicial" => "877"
                        "paginaFinal" => "885"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9768945"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/15792129/0000004300000010/v1_201305150259/S1579212907601256/v1_201305150259/en/main.assets"
  "Apartado" => null
  "PDF" => "https://static.elsevier.es/multimedia/15792129/0000004300000010/v1_201305150259/S1579212907601256/v1_201305150259/en/main.pdf?idApp=UINPBA00003Z&text.app=https://archbronconeumol.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907601256?idApp=UINPBA00003Z"
]
Share
Journal Information
Vol. 43. Issue 10.
Pages 542-548 (January 2007)
Share
Share
Download PDF
More article options
Vol. 43. Issue 10.
Pages 542-548 (January 2007)
Original Articles
Full text access
Interleukin-8 Expression in Lung Tissue During Ischemia-Reperfusion
Visits
3976
José María Matillaa,
Corresponding author
jmmatilla17@hotmail.com

Correspondence: Dr. J.M. Matilla González. Servicio de Cirugía Torácica. Hospital Clínico Universitario de Valladolid. Ramón y Cajal, 3. 47005 Valladolid. España
, Mariano García-Yustea, Mariano Sánchez Crespob, Manuel J. Gayoso, Félix Herasa, Marta Jiménez Pradaa, Andrés Alonsob, Guillermo Ramosa
a Unidad de Cirugía Torácica Experimental, Facultad de Medicina, Universidad de Valladolid, Valladolid, Spain
b Instituto de Biología y Genética Molecular (IBGM), Universidad de Valladolid-CSIC, Valladolid, Spain
This item has received
Article information
Abstract
Bibliography
Download PDF
Statistics
Objective

Local cytokine production is a pathogenic factor in ischemia-reperfusion injury in early graft dysfunction. This study analyzed interleukin 8 (IL-8) messenger RNA (mRNA) expression in lung tissue and the association between IL-8 mRNA levels and interstitial lung changes in an experimental model of warm lung ischemia-reperfusion.

MATERIAL AND METHODS

We studied 16 New Zealand rabbits divided into 3 groups: control, ischemia (tissue taken from right lower lobe after 1, 2, or 3 hours of ischemia), and reperfusion (tissue taken from right upper and middle lobes after 1 hour of ischemia and 1, 2, or 3 hours of reperfusion). Expression of IL-8 mRNA was determined by reverse transcription and polymerase chain reaction. Interstitial infiltration by polymorphonuclear neutrophils was determined. The Mann-Whitney U-test was used for statistical comparisons, with P<.05 considered to indicate a significant result.

RESULTS

During ischemia, IL-8 mRNA levels were elevated at the end of hour 1 (P=.009) with respect to the control group, but not thereafter. Interstitial changes were minimal. IL-8 mRNA levels during reperfusion were similar to those observed during ischemia, with a slight increase at the end of hour 2. There were no significant differences between hours 1, 2, and 3. Polymorphonuclear neutrophil recruitment occurred at the beginning of reperfusion (P=.014), but no significant differences were observed at hours 2 or 3. Progressive thickening of alveolar septa and edema was documented.

CONCLUSIONS

Changes in IL-8 mRNA expression during ischemia precede interstitial infiltration by polymorphonuclear neutrophils during reperfusion, suggesting that the 2 processes are related. Quantification of IL-8 mRNA expression could facilitate early diagnosis of graft dysfunction.

Key words:
Ischemia-reperfusion
Interleukin 8
Polymorphonuclear leukocytes
Lung transplantation
Objetivo

La generación local de citocinas es un factor patogénico en el daño por isquemia-reperfusión en la disfunción precoz del injerto. Este estudio analiza la expresión en tejido pulmonar de ARN mensajero de interleucina-8 (ARNm de IL-8) y su relación con los cambios intersticiales pulmonares en un modelo experimental de isquemia-reperfusión pulmonar normotérmica.

MATERIAL Y MÉTODOS

Se estudiaron 16 conejos de la raza Nueva Zelanda en 3 grupos de estudio: a) basal; b) isquemia (lóbulo inferior derecho tras isquemia de 1, 2 o 3 h), y c) reperfusión (lóbulos superior y medio derechos tras 1 h de isquemia y 1, 2 o 3 h de reperfusión). Se deter- minó la expresión del ARNm de IL-8 mediante transcripción inversa y reacción en cadena de la polimerasa y estudió la infiltración intersticial por polimorfonucleares (PMN). Para el análisis estadístico se empleó el test de la U de Mann-Whitney aceptando como significativo un valor de p < 0,05.

RESULTADOS

Durante el período de isquemia se observó respecto al basal elevación del ARNm de IL-8 al final de la primera hora (p = 0,009), pero no durante el resto del período isquémico. Los cambios intersticiales fueron mínimos. Durante la reperfusión los valores de ARNm de IL-8 fueron semejantes a los observados durante la isquemia, con una ligera elevación al final de segunda hora; no hubo diferencias significativas entre la primera, segunda y tercera horas. Hubo reclutamiento de PMN al inicio de la reperfusión (p = 0,014), sin observarse diferencias significativas en la segunda y tercera horas. Se objetivó un engrosamiento progresivo de los tabiques interalveolares y edema.

CONCLUSIONES

Los cambios en la expresión del ARNm de IL-8 durante la isquemia preceden a la infiltración intersticial de PMN durante la reperfusión, lo que señala una relación entre ambos procesos. La cuantificación del ARNm de IL-8 podría ser un procedimiento para el seguimiento diagnóstico de la disfunción precoz del injerto.

Palabras clave:
Isquemia-reperfusión
Interleucina-8
Leucocitos polimorfonucleares
Trasplante pulmonar
Full text is only aviable in PDF
REFERENCES
[1]
H Mal, M Dehoux, C Sleiman, J Boczkowski, G Leséche, R Pariente, et al.
Early release of proinflammatory cytokines after lung transplantation.
Chest, 113 (1998), pp. 645-651
[2]
mL Jones, MS Mulligan, CM Flory, PA Ward, JS Warren.
Potential role of monocyte chemoattractant protein 1/JE in monocyte/ macrophage-dependent IgA immune complex alveolitis in the rat.
J Immunol, 149 (1992), pp. 2147-2154
[3]
TP Shanley, H Schmal, RL Warner, E Schmid, HP Friedl, PA Ward.
Requirement for C-X-C chemokines (macrophage inflammatory protein-2, and cytokine-induced neutrophil chemoattractant) in IgG immune complex-induced lung injury.
J Immunol, 158 (1997), pp. 3439-3448
[4]
BV Naidu, B Krishnadasan, K Byrne, AL Farr, M Rosengart, ED Verrier, et al.
Regulation of chemokine expression by cyclosporine A in alveolar macrophages exposed to hypoxia and reoxygenation.
Ann Thorac Surg, 74 (2002), pp. 899-905
[5]
BF Meyer, J Lynch, EP Trulock, TJ Guthrie, JD Cooper, GA Patterson.
Lung transplantation: a decade of experience.
Ann Surg, 230 (1999), pp. 362-367
[6]
JM Schröder, U Mrowietz, E Christophers.
Purification and partial biologic characterization of a human lymphocyte derived peptide with potent neutrophil stimulating activity.
J Immunol, 140 (1988), pp. 3534-3540
[7]
AP Metinko, SL Kunkel, TJ Standiford, RM Strieter.
Anoxia-hyperoxia induces monocyte derived interleukin-8.
J Clin Invest, 90 (1992), pp. 791-798
[8]
I Nagahiro, M Aoe, M Yamashita, H Date, A Andou, N Shimizu.
EPC-K1 is effective in lung preservation in a ex vivo rabbit lung perfusion model.
Ann Thorac Surg, 63 (1997), pp. 954-959
[9]
M Haniuda, CM Dresler, S Hasegawa, GA Patterson, JD Cooper.
Changes in vascular permeability with ischemic time, temperature, and inspired oxygen fraction in isolated rabbit lungs.
Ann Thorac Surg, 57 (1994), pp. 708-714
[10]
M Haniuda, S Hasegawa, T Shiraishi, CM Dresler, JD Cooper, GA Patterson.
Effects of inflammation volume during lung preservation on pulmonary capillary permeability.
J Thorac Cardiovasc Surg, 112 (1996), pp. 85-93
[11]
JM Schröder, U Mrowietz, E Christophers.
Purification and partial biologic characterization of a human lymphocyte derived peptide with potent neutrophil stimulating activity.
J Immunol, 140 (1988), pp. 3534-3540
[12]
DW Harkin, AB Barros, K McCallion, M Hoper, MI Halliday, FC Campbell.
Bactericidal/permeability-increasing protein attenuates systemic inflammation and acute lung injury in porcine lower limb ischemia-reperfusion injury.
Ann Surg, 234 (2001), pp. 233-244
[13]
AR Mueller, KP Platz, M Haak, H Undi, C Muller, E Kottgen, et al.
The release of cytokines, adhesion molecules and extracellular matrix parameters during and after reperfusion in human liver transplantation.
Transplantation, 62 (1996), pp. 1118-1126
[14]
N Sekido, N Mukaida, A Harada, I Nakanishi, Y Watanabe, K Matsushima.
Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8.
Nature, 365 (1993), pp. 654-657
[15]
T Sakuma, K Takahashi, N Ohya, O Kajikawa, TR Martin, AMA Matthay.
Ischemia-reperfusion lung injury in rabbits: mechanisms of injury and protection.
Am J Physiol Lung Cell Mol Physiol, 276 (1999), pp. L137-LL45
[16]
M de Perrot, Y Sekine, S Fischer, TK Waddell, K McRae, M Liu, et al.
Interleukin-8 release during early reperfusion predicts graft function in human lung transplantation.
Am J Respir Crit Care Med, 165 (2002), pp. 211-215
[17]
AJ Fisher, SC Donnelly, N Hirani, C Haslett, RM Strieter, JH Dark, et al.
Elevated levels of interleukin-8 in donor lungs is associated with early graft failure after lung transplantation.
Am J Respir Crit Care Med, 63 (2001), pp. 259-265
[18]
H Kishima, SI Takeda, S Miyoshi, A Matsumura, M Minami, T Utsumi, et al.
Microvascular permeability of the non-heart beating rabbit lung after warm ischemia and reperfusion: role of neutrophil elastase.
Ann Thorac Surg, 65 (1998), pp. 913-918
[19]
R Adoumie, C Serrick, A Giaid, H Shennib.
Early cellular events in the lung allograft.
Ann Thorac Surg, 54 (1992), pp. 1071-1077
[20]
CN Steimle, TD Guynn, mL Morganroth, SF Bolling, K Carr, M Deeb.
Neutrophils are not necessary for ischemia-reperfusion lung injury.
Ann Thorac Surg, 53 (1992), pp. 64-73
[21]
M de Perrot, M Liu, TK Waddell, S Keshavjee.
Ischemia-reperfusion induced lung injury.
Am J Respir Crit Care Med, 167 (2003), pp. 490-511
[22]
AO Halldorsson, M Kronon, BS Allen, S Rahman, T Wang, M Layland, et al.
Controlled reperfusion prevents pulmonary injury after 24 hours of lung preservation.
Ann Thorac Surg, 66 (1998), pp. 877-885

This study was realized with the scientific help and the financiation of the Sociedad Española de Neumología y Cirugía Torácica. (SEPAR).

Copyright © 2007. Sociedad Española de Neumología y Cirugía Torácica (SEPAR)
Archivos de Bronconeumología
Article options
Tools

Are you a health professional able to prescribe or dispense drugs?