Correspondence: Dr. J.M. Matilla González. Servicio de Cirugía Torácica. Hospital Clínico Universitario de Valladolid. Ramón y Cajal, 3. 47005 Valladolid. España
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Pérez-Yarza, Nicolás Cobos, Juan José de la Cruz" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Eduardo G." "apellidos" => "Pérez-Yarza" ] 1 => array:2 [ "nombre" => "Nicolás" "apellidos" => "Cobos" ] 2 => array:2 [ "nombre" => "Juan José" "apellidos" => "de la Cruz" ] 3 => array:1 [ "colaborador" => "on behalf of the Asthma Working Group of the Spanish Society of Pediatric Pneumology" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907601244?idApp=UINPBA00003Z" "url" => "/15792129/0000004300000010/v1_201305150259/S1579212907601244/v1_201305150259/en/main.assets" ] "en" => array:15 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>" "titulo" => "Interleukin-8 Expression in Lung Tissue During Ischemia-Reperfusion" "tieneTextoCompleto" => 0 "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "542" "paginaFinal" => "548" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "José María Matilla, Mariano García-Yuste, Mariano Sánchez Crespo, Manuel J. Gayoso, Félix Heras, Marta Jiménez Prada, Andrés Alonso, Guillermo Ramos" "autores" => array:8 [ 0 => array:4 [ "nombre" => "José María" "apellidos" => "Matilla" "email" => array:1 [ 0 => "jmmatilla17@hotmail.com" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff1" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor1" ] ] ] 1 => array:3 [ "nombre" => "Mariano" "apellidos" => "García-Yuste" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff1" ] ] ] 2 => array:3 [ "nombre" => "Mariano Sánchez" "apellidos" => "Crespo" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff2" ] ] ] 3 => array:2 [ "nombre" => "Manuel J." "apellidos" => "Gayoso" ] 4 => array:3 [ "nombre" => "Félix" "apellidos" => "Heras" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff1" ] ] ] 5 => array:3 [ "nombre" => "Marta Jiménez" "apellidos" => "Prada" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff1" ] ] ] 6 => array:3 [ "nombre" => "Andrés" "apellidos" => "Alonso" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff2" ] ] ] 7 => array:3 [ "nombre" => "Guillermo" "apellidos" => "Ramos" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff1" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Unidad de Cirugía Torácica Experimental, Facultad de Medicina, Universidad de Valladolid, Valladolid, Spain" "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff1" ] 1 => array:3 [ "entidad" => "Instituto de Biología y Genética Molecular (IBGM), Universidad de Valladolid-CSIC, Valladolid, Spain" "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff2" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor1" "etiqueta" => "*" "correspondencia" => "Correspondence: Dr. J.M. Matilla González. Servicio de Cirugía Torácica. Hospital Clínico Universitario de Valladolid. Ramón y Cajal, 3. 47005 Valladolid. España" ] ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2004-08-16" "fechaAceptado" => "2006-10-24" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Key words" "identificador" => "xpalclavsec154621" "palabras" => array:4 [ 0 => "Ischemia-reperfusion" 1 => "Interleukin 8" 2 => "Polymorphonuclear leukocytes" 3 => "Lung transplantation" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec154622" "palabras" => array:4 [ 0 => "Isquemia-reperfusión" 1 => "Interleucina-8" 2 => "Leucocitos polimorfonucleares" 3 => "Trasplante pulmonar" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:1 [ "resumen" => "<span class="elsevierStyleSectionTitle">Objective</span><p class="elsevierStyleSimplePara elsevierViewall">Local cytokine production is a pathogenic factor in ischemia-reperfusion injury in early graft dysfunction. This study analyzed interleukin 8 (IL-8) messenger RNA (mRNA) expression in lung tissue and the association between IL-8 mRNA levels and interstitial lung changes in an experimental model of warm lung ischemia-reperfusion.</p> <span class="elsevierStyleSectionTitle">MATERIAL AND METHODS</span><p class="elsevierStyleSimplePara elsevierViewall">We studied 16 New Zealand rabbits divided into 3 groups: control, ischemia (tissue taken from right lower lobe after 1, 2, or 3 hours of ischemia), and reperfusion (tissue taken from right upper and middle lobes after 1 hour of ischemia and 1, 2, or 3 hours of reperfusion). Expression of IL-8 mRNA was determined by reverse transcription and polymerase chain reaction. Interstitial infiltration by polymorphonuclear neutrophils was determined. The Mann-Whitney U-test was used for statistical comparisons, with <span class="elsevierStyleItalic">P</span><.05 considered to indicate a significant result.</p> <span class="elsevierStyleSectionTitle">RESULTS</span><p class="elsevierStyleSimplePara elsevierViewall">During ischemia, IL-8 mRNA levels were elevated at the end of hour 1 (<span class="elsevierStyleItalic">P</span>=.009) with respect to the control group, but not thereafter. Interstitial changes were minimal. IL-8 mRNA levels during reperfusion were similar to those observed during ischemia, with a slight increase at the end of hour 2. There were no significant differences between hours 1, 2, and 3. Polymorphonuclear neutrophil recruitment occurred at the beginning of reperfusion (<span class="elsevierStyleItalic">P</span>=.014), but no significant differences were observed at hours 2 or 3. Progressive thickening of alveolar septa and edema was documented.</p> <span class="elsevierStyleSectionTitle">CONCLUSIONS</span><p class="elsevierStyleSimplePara elsevierViewall">Changes in IL-8 mRNA expression during ischemia precede interstitial infiltration by polymorphonuclear neutrophils during reperfusion, suggesting that the 2 processes are related. Quantification of IL-8 mRNA expression could facilitate early diagnosis of graft dysfunction.</p>" ] "es" => array:1 [ "resumen" => "<span class="elsevierStyleSectionTitle">Objetivo</span><p class="elsevierStyleSimplePara elsevierViewall">La generación local de citocinas es un factor patogénico en el daño por isquemia-reperfusión en la disfunción precoz del injerto. Este estudio analiza la expresión en tejido pulmonar de ARN mensajero de interleucina-8 (ARNm de IL-8) y su relación con los cambios intersticiales pulmonares en un modelo experimental de isquemia-reperfusión pulmonar normotérmica.</p> <span class="elsevierStyleSectionTitle">MATERIAL Y MÉTODOS</span><p class="elsevierStyleSimplePara elsevierViewall">Se estudiaron 16 conejos de la raza Nueva Zelanda en 3 grupos de estudio: a) basal; b) isquemia (lóbulo inferior derecho tras isquemia de 1, 2 o 3 h), y c) reperfusión (lóbulos superior y medio derechos tras 1 h de isquemia y 1, 2 o 3 h de reperfusión). Se deter- minó la expresión del ARNm de IL-8 mediante transcripción inversa y reacción en cadena de la polimerasa y estudió la infiltración intersticial por polimorfonucleares (PMN). Para el análisis estadístico se empleó el test de la U de Mann-Whitney aceptando como significativo un valor de p < 0,05.</p> <span class="elsevierStyleSectionTitle">RESULTADOS</span><p class="elsevierStyleSimplePara elsevierViewall">Durante el período de isquemia se observó respecto al basal elevación del ARNm de IL-8 al final de la primera hora (p = 0,009), pero no durante el resto del período isquémico. Los cambios intersticiales fueron mínimos. Durante la reperfusión los valores de ARNm de IL-8 fueron semejantes a los observados durante la isquemia, con una ligera elevación al final de segunda hora; no hubo diferencias significativas entre la primera, segunda y tercera horas. Hubo reclutamiento de PMN al inicio de la reperfusión (p = 0,014), sin observarse diferencias significativas en la segunda y tercera horas. Se objetivó un engrosamiento progresivo de los tabiques interalveolares y edema.</p> <span class="elsevierStyleSectionTitle">CONCLUSIONES</span><p class="elsevierStyleSimplePara elsevierViewall">Los cambios en la expresión del ARNm de IL-8 durante la isquemia preceden a la infiltración intersticial de PMN durante la reperfusión, lo que señala una relación entre ambos procesos. La cuantificación del ARNm de IL-8 podría ser un procedimiento para el seguimiento diagnóstico de la disfunción precoz del injerto.</p>" ] ] "NotaPie" => array:1 [ 0 => array:1 [ "nota" => "<p class="elsevierStyleNotepara">This study was realized with the scientific help and the financiation of the Sociedad Española de Neumología y Cirugía Torácica. (SEPAR).</p>" ] ] "bibliografia" => array:2 [ "titulo" => "REFERENCES" "seccion" => array:1 [ 0 => array:1 [ "bibliografiaReferencia" => array:22 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Early release of proinflammatory cytokines after lung transplantation" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "H Mal" 1 => "M Dehoux" 2 => "C Sleiman" 3 => "J Boczkowski" 4 => "G Leséche" 5 => "R Pariente" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Chest" "fecha" => "1998" "volumen" => "113" "paginaInicial" => "645" "paginaFinal" => "651" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9515837" "web" => "Medline" ] ] ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib2" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ 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Year/Month | Html | Total | |
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2024 November | 4 | 3 | 7 |
2024 October | 32 | 23 | 55 |
2024 September | 31 | 12 | 43 |
2024 August | 40 | 33 | 73 |
2024 July | 23 | 21 | 44 |
2024 June | 31 | 23 | 54 |
2024 May | 33 | 33 | 66 |
2024 April | 18 | 24 | 42 |
2024 March | 15 | 13 | 28 |
2024 February | 17 | 19 | 36 |
2023 March | 3 | 1 | 4 |
2023 February | 19 | 25 | 44 |
2023 January | 18 | 36 | 54 |
2022 December | 16 | 29 | 45 |
2022 November | 17 | 25 | 42 |
2022 October | 22 | 30 | 52 |
2022 September | 16 | 26 | 42 |
2022 August | 25 | 35 | 60 |
2022 July | 17 | 34 | 51 |
2022 June | 14 | 35 | 49 |
2022 May | 22 | 34 | 56 |
2022 April | 16 | 32 | 48 |
2022 March | 22 | 34 | 56 |
2022 February | 14 | 19 | 33 |
2022 January | 14 | 36 | 50 |
2021 December | 18 | 55 | 73 |
2021 November | 15 | 45 | 60 |
2021 October | 21 | 48 | 69 |
2021 September | 15 | 63 | 78 |
2021 August | 11 | 30 | 41 |
2021 July | 12 | 18 | 30 |
2021 June | 9 | 20 | 29 |
2021 May | 16 | 34 | 50 |
2021 April | 32 | 64 | 96 |
2021 March | 31 | 20 | 51 |
2021 February | 12 | 18 | 30 |
2021 January | 12 | 14 | 26 |
2020 December | 7 | 35 | 42 |
2020 November | 16 | 15 | 31 |
2020 October | 5 | 6 | 11 |
2020 September | 7 | 3 | 10 |
2020 August | 12 | 6 | 18 |
2020 July | 14 | 15 | 29 |
2020 June | 5 | 6 | 11 |
2020 May | 8 | 13 | 21 |
2020 April | 12 | 10 | 22 |
2020 March | 7 | 10 | 17 |
2020 February | 13 | 10 | 23 |
2020 January | 13 | 18 | 31 |
2019 December | 12 | 8 | 20 |
2019 November | 6 | 7 | 13 |
2019 October | 6 | 4 | 10 |
2019 September | 6 | 5 | 11 |
2019 August | 10 | 8 | 18 |
2019 July | 14 | 15 | 29 |
2019 June | 3 | 4 | 7 |
2019 May | 12 | 7 | 19 |
2019 April | 26 | 13 | 39 |
2019 March | 11 | 8 | 19 |
2019 February | 4 | 14 | 18 |
2019 January | 5 | 11 | 16 |
2018 December | 10 | 15 | 25 |
2018 November | 13 | 7 | 20 |
2018 October | 16 | 19 | 35 |
2018 September | 11 | 3 | 14 |
2018 May | 4 | 0 | 4 |
2018 April | 9 | 7 | 16 |
2018 March | 6 | 1 | 7 |
2018 February | 5 | 10 | 15 |
2018 January | 3 | 6 | 9 |
2017 December | 2 | 8 | 10 |
2017 November | 4 | 8 | 12 |
2017 October | 5 | 11 | 16 |
2017 September | 11 | 15 | 26 |
2017 August | 4 | 16 | 20 |
2017 July | 5 | 11 | 16 |
2017 June | 8 | 12 | 20 |
2017 May | 6 | 10 | 16 |
2017 April | 15 | 9 | 24 |
2017 March | 26 | 44 | 70 |
2017 February | 1 | 2 | 3 |
2017 January | 1 | 2 | 3 |
2016 December | 11 | 3 | 14 |
2016 November | 9 | 9 | 18 |
2016 October | 7 | 13 | 20 |
2016 September | 3 | 4 | 7 |
2016 August | 6 | 5 | 11 |
2016 April | 1 | 0 | 1 |
2016 March | 1 | 0 | 1 |
2015 December | 3 | 0 | 3 |
2015 October | 34 | 14 | 48 |
2015 September | 21 | 13 | 34 |
2015 August | 14 | 20 | 34 |
2015 July | 36 | 12 | 48 |
2015 June | 26 | 8 | 34 |
2015 May | 35 | 20 | 55 |
2015 April | 17 | 13 | 30 |
2015 March | 25 | 10 | 35 |
2015 February | 39 | 6 | 45 |
2015 January | 24 | 5 | 29 |
2014 December | 22 | 4 | 26 |
2014 November | 23 | 5 | 28 |
2014 October | 30 | 10 | 40 |
2014 September | 24 | 8 | 32 |
2014 August | 33 | 10 | 43 |
2014 July | 22 | 6 | 28 |
2014 June | 34 | 8 | 42 |
2014 May | 42 | 10 | 52 |
2014 April | 39 | 2 | 41 |
2014 March | 49 | 12 | 61 |
2014 February | 42 | 6 | 48 |
2014 January | 34 | 6 | 40 |
2013 December | 48 | 10 | 58 |
2013 November | 42 | 2 | 44 |
2013 October | 36 | 8 | 44 |
2013 September | 26 | 9 | 35 |
2013 August | 42 | 4 | 46 |
2013 July | 51 | 14 | 65 |
2013 June | 27 | 6 | 33 |
2013 May | 41 | 6 | 47 |
2013 April | 11 | 4 | 15 |
2013 March | 6 | 1 | 7 |