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Vol. 40. Issue 10.
Pages 438-442 (October 2004)
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Vol. 40. Issue 10.
Pages 438-442 (October 2004)
Original Articles
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Lung Transplantation in Rats: a Viable Experimental Model
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N. Santana Rodrígueza,
Corresponding author
norbesr@terra.es

Correspondence: Dr. N. Santana Rodríguez. Unidad de Cirugía Torácica. Clínica San Roque. C/ Juan E. Doreste, 11. 35001 Las Palmas de Gran Canaria. España
, J.L. Martín Barrasab, A. López Garcíac, P. Rodríguez Suárezb, M. Ponce Gonzálezb, J. Freixinet Gilartb
a Unidad de Cirugía Torácica, Clínica San Roque, Las Palmas de Gran Canaria, Spain
b Unidad de Cirugía Torácica, Unidad de Investigación, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain
c Servicio de Anatomía Patológica, Hospital Universitario Puerta de Hierro, Madrid, Spain
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Objectives

TO incorporate a new fast, safe, and reversible anesthetic procedure into the experimental model of lung transplantation (LT) using a cuff technique originally described by Mizuta.

Material and Method

Eighty-eight Sprague-Dawley rats were used in the experimental model. Thirty left LTs were performed, using 60 rats. The donor heart-lung block was excised by median sternotomy with dissection of the left lung and cuffs (intravenous catheters cut into 3-mm sections) were put in place. The left lung was implanted in the recipient by lateral thoracotomy using the cuffs for anastomoses. The duration of surgery and postoperative complications were recorded. Also noted were signs of ischemia-reperfusion injury, and acute rejection of the transplanted lung.

Results

We discarded lungs excised from 8 animals when developing the experimental model. Transplants could not be completed in 10 rats due to technical problems, despite satisfactory excision. Of the rats who received a transplant, 4 died in the first 24 hours and 26 survived to 48 hours. They were then killed and examined. The state of the anastomoses was good and signs of ischemia-reperfusion injury, as well as acute rejection were observed in the parenchyma of the transplanted lung.

Conclusions

LT with cuffs in rats is a valid, reliable, reproducible, and cheap model for studying ischemia-reperfusion injury and rejection in LT. The surgical technique is complex, requiring experienced surgeons and a long learning process. Modification of the technique to more closely resemble the surgical procedure in humans is possible, thus facilitating interpretation and allowing more reliable extrapolation to humans.

Key Words:
Lung transplant
Experimental surgery
Experimental transplant
Objetivos

Desarrollar el modelo experimental de trasplante pulmonar (TP) con cuffs (técnica de manguito), inicialmente descrito por Mizuta, como medio de estudio del TP en nuestro medio incorporando un nuevo protocolo anestésico rápido, seguro y reversible.

Material Y Método

Se han utilizado ratas Sprague-Dawley (n = 88) para el desarrollo del modelo experimental y se han logrado realizar 30 TP izquierdos (n = 60). En el animal donante se han efectuado una esternotomía media y extracción del bloque cardiopulmonar con disección del pulmón izquierdo y colocación de los cuffs (catéteres de venopunción cortados en cilindros de 3 mm). En el receptor, a través de una toracotomía lateral, se ha llevado a cabo el implante del pulmón izquierdo mediante las anastomosis con cuffs. Se han valorado el tiempo quirúrgico y las complicaciones postoperatorias, así como la presencia de signos de lesión de isquemia-reperfusión y rechazo agudo en el pulmón trasplantado.

Resultados

Durante el desarrollo del modelo experimental rechazamos a 8 animales en la extracción. Tras com-pletarla satisfactoriamente, no se pudo realizar el implante en 10 ratas por problemas técnicos. De los animales trasplantados, 4 fallecieron en las primeras 24 h y 26 ratas sobrevivieron hasta las 48 h, momento en que fueron sacrificadas. Hallamos un buen estado de las anastomosis y signos de lesión de isquemia-reperfusión y rechazo agudo en el parénquima pulmonar del pulmón trasplantado.

Conclusiones

El TP con cuffs en ratas es un modelo válido, fiable, reproducible y económico para el estudio del TP y de los fenómenos de isquemia-reperfusión y rechazo. La técnica quirúrgica es compleja, tiene una larga curva de aprendizaje y requiere personal con experiencia quirúrgica. Además, es subsidiaria de modificaciones que hagan que se asemeje más a la técnica quirúrgica utilizada en clínica humana para interpretar mejor y poder extrapolar los resultados al humano con más fiabilidad.

Palabras clave:
Trasplante pulmonar
Cirugía experimental
Trasplante experimental
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This project was funded by the Health Research Fund (FIS).

Copyright © 2004. Sociedad Española de Neumología y Cirugía Torácica (SEPAR)
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