array:23 [
  "pii" => "S1579212906603522"
  "issn" => "15792129"
  "doi" => "10.1016/S1579-2129(06)60352-2"
  "estado" => "S300"
  "fechaPublicacion" => "2004-10-01"
  "aid" => "60352"
  "copyright" => "Sociedad Española de Neumología y Cirugía Torácica (SEPAR)"
  "copyrightAnyo" => "2004"
  "documento" => "article"
  "crossmark" => 0
  "subdocumento" => "fla"
  "cita" => "Arch Bronconeumol. 2004;40:438-42"
  "abierto" => array:3 [
    "ES" => false
    "ES2" => false
    "LATM" => false
  ]
  "gratuito" => false
  "lecturas" => array:2 [
    "total" => 3001
    "formatos" => array:3 [
      "EPUB" => 101
      "HTML" => 2142
      "PDF" => 758
    ]
  ]
  "itemSiguiente" => array:18 [
    "pii" => "S1579212906603534"
    "issn" => "15792129"
    "doi" => "10.1016/S1579-2129(06)60353-4"
    "estado" => "S300"
    "fechaPublicacion" => "2004-10-01"
    "aid" => "60353"
    "copyright" => "Sociedad Española de Neumología y Cirugía Torácica (SEPAR)"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Arch Bronconeumol. 2004;40:443-8"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 2316
      "formatos" => array:3 [
        "EPUB" => 96
        "HTML" => 1566
        "PDF" => 654
      ]
    ]
    "en" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
      "titulo" => "Four-Year Results After Lung Volume Reduction Surgery for Emphysema"
      "tienePdf" => "en"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "443"
          "paginaFinal" => "448"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "G&#46; Juan Samper, M&#46; Ram&#243;n Capilla, A&#46; Cant&#243; Armengol, M&#46;L&#46; Mart&#237;nez P&#233;rez, T&#46; Lloret P&#233;rez, E&#46; Rubio Gomis, J&#46; Mar&#237;n Pardo"
          "autores" => array:7 [
            0 => array:3 [
              "preGrado" => "Prof"
              "nombre" => "G&#46;"
              "apellidos" => "Juan Samper"
            ]
            1 => array:2 [
              "nombre" => "M&#46;"
              "apellidos" => "Ram&#243;n Capilla"
            ]
            2 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Cant&#243; Armengol"
            ]
            3 => array:2 [
              "nombre" => "M&#46;L&#46;"
              "apellidos" => "Mart&#237;nez P&#233;rez"
            ]
            4 => array:2 [
              "nombre" => "T&#46;"
              "apellidos" => "Lloret P&#233;rez"
            ]
            5 => array:2 [
              "nombre" => "E&#46;"
              "apellidos" => "Rubio Gomis"
            ]
            6 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Mar&#237;n Pardo"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212906603534?idApp=UINPBA00003Z"
    "url" => "/15792129/0000004000000010/v1_201305141820/S1579212906603534/v1_201305141820/en/main.assets"
  ]
  "itemAnterior" => array:18 [
    "pii" => "S1579212906603510"
    "issn" => "15792129"
    "doi" => "10.1016/S1579-2129(06)60351-0"
    "estado" => "S300"
    "fechaPublicacion" => "2004-10-01"
    "aid" => "60351"
    "copyright" => "Sociedad Espa&#241;ola de Neumolog&#237;a y Cirug&#237;a Tor&#225;cica &#40;SEPAR&#41;"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Arch Bronconeumol. 2004;40:431-7"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 2796
      "formatos" => array:3 [
        "EPUB" => 96
        "HTML" => 1975
        "PDF" => 725
      ]
    ]
    "en" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
      "titulo" => "Quality of Life With Chronic Obstructive Pulmonary Disease&#58; the Influence of Level of Patient Care"
      "tienePdf" => "en"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "431"
          "paginaFinal" => "437"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "J&#46; de Miguel D&#237;ez, J&#46;L&#46; Izquierdo Alonso, J&#46;M&#46; Rodr&#237;guez Gonz&#225;lez-Moro, P&#46; de Lucas Ramos, J&#46;M&#46; Bell&#243;n Cano, J&#46; Molina Par&#237;s"
          "autores" => array:6 [
            0 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "de Miguel D&#237;ez"
            ]
            1 => array:3 [
              "preGrado" => "Dr&#46;"
              "nombre" => "J&#46;L&#46;"
              "apellidos" => "Izquierdo Alonso"
            ]
            2 => array:2 [
              "nombre" => "J&#46;M&#46;"
              "apellidos" => "Rodr&#237;guez Gonz&#225;lez-Moro"
            ]
            3 => array:2 [
              "nombre" => "P&#46;"
              "apellidos" => "de Lucas Ramos"
            ]
            4 => array:2 [
              "nombre" => "J&#46;M&#46;"
              "apellidos" => "Bell&#243;n Cano"
            ]
            5 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Molina Par&#237;s"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212906603510?idApp=UINPBA00003Z"
    "url" => "/15792129/0000004000000010/v1_201305141820/S1579212906603510/v1_201305141820/en/main.assets"
  ]
  "en" => array:15 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
    "titulo" => "Lung Transplantation in Rats&#58; a Viable Experimental Model"
    "tieneTextoCompleto" => 0
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "438"
        "paginaFinal" => "442"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "N&#46; Santana Rodr&#237;guez, J&#46;L&#46; Mart&#237;n Barrasa, A&#46; L&#243;pez Garc&#237;a, P&#46; Rodr&#237;guez Su&#225;rez, M&#46; Ponce Gonz&#225;lez, J&#46; Freixinet Gilart"
        "autores" => array:6 [
          0 => array:5 [
            "preGrado" => "Dr&#46;"
            "nombre" => "N&#46;"
            "apellidos" => "Santana Rodr&#237;guez"
            "email" => array:1 [
              0 => "norbesr&#64;terra&#46;es"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor1"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "J&#46;L&#46;"
            "apellidos" => "Mart&#237;n Barrasa"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "A&#46;"
            "apellidos" => "L&#243;pez Garc&#237;a"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff3"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "P&#46;"
            "apellidos" => "Rodr&#237;guez Su&#225;rez"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          4 => array:3 [
            "nombre" => "M&#46;"
            "apellidos" => "Ponce Gonz&#225;lez"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          5 => array:3 [
            "nombre" => "J&#46;"
            "apellidos" => "Freixinet Gilart"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:3 [
          0 => array:3 [
            "entidad" => "Unidad de Cirug&#237;a Tor&#225;cica&#44; Cl&#237;nica San Roque&#44; Las Palmas de Gran Canaria&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "aff1"
          ]
          1 => array:3 [
            "entidad" => "Unidad de Cirug&#237;a Tor&#225;cica&#44; Unidad de Investigaci&#243;n&#44; Hospital Universitario Dr&#46; Negr&#237;n&#44; Las Palmas de Gran Canaria&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "aff2"
          ]
          2 => array:3 [
            "entidad" => "Servicio de Anatom&#237;a Patol&#243;gica&#44; Hospital Universitario Puerta de Hierro&#44; Madrid&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">c</span>"
            "identificador" => "aff3"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor1"
            "etiqueta" => "&#42;"
            "correspondencia" => "Correspondence&#58; Dr&#46; N&#46; Santana Rodr&#237;guez&#46; Unidad de Cirug&#237;a Tor&#225;cica&#46; Cl&#237;nica San Roque&#46; C&#47; Juan E&#46; Doreste&#44; 11&#46; 35001 Las Palmas de Gran Canaria&#46; Espa&#241;a"
          ]
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2003-11-13"
    "fechaAceptado" => "2004-03-23"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Key Words"
          "identificador" => "xpalclavsec153122"
          "palabras" => array:3 [
            0 => "Lung transplant"
            1 => "Experimental surgery"
            2 => "Experimental transplant"
          ]
        ]
      ]
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec153123"
          "palabras" => array:3 [
            0 => "Trasplante pulmonar"
            1 => "Cirug&#237;a experimental"
            2 => "Trasplante experimental"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:1 [
        "resumen" => "<span class="elsevierStyleSectionTitle">Objectives</span><p class="elsevierStyleSimplePara elsevierViewall">TO incorporate a new fast&#44; safe&#44; and reversible anesthetic procedure into the experimental model of lung transplantation &#40;LT&#41; using a cuff technique originally described by Mizuta&#46;</p> <span class="elsevierStyleSectionTitle">Material and Method</span><p class="elsevierStyleSimplePara elsevierViewall">Eighty-eight Sprague-Dawley rats were used in the experimental model&#46; Thirty left LTs were performed&#44; using 60 rats&#46; The donor heart-lung block was excised by median sternotomy with dissection of the left lung and cuffs &#40;intravenous catheters cut into 3-mm sections&#41; were put in place&#46; The left lung was implanted in the recipient by lateral thoracotomy using the cuffs for anastomoses&#46; The duration of surgery and postoperative complications were recorded&#46; Also noted were signs of ischemia-reperfusion injury&#44; and acute rejection of the transplanted lung&#46;</p> <span class="elsevierStyleSectionTitle">Results</span><p class="elsevierStyleSimplePara elsevierViewall">We discarded lungs excised from 8 animals when developing the experimental model&#46; Transplants could not be completed in 10 rats due to technical problems&#44; despite satisfactory excision&#46; Of the rats who received a transplant&#44; 4 died in the first 24 hours and 26 survived to 48 hours&#46; They were then killed and examined&#46; The state of the anastomoses was good and signs of ischemia-reperfusion injury&#44; as well as acute rejection were observed in the parenchyma of the transplanted lung&#46;</p> <span class="elsevierStyleSectionTitle">Conclusions</span><p class="elsevierStyleSimplePara elsevierViewall">LT with cuffs in rats is a valid&#44; reliable&#44; reproducible&#44; and cheap model for studying ischemia-reperfusion injury and rejection in LT&#46; The surgical technique is complex&#44; requiring experienced surgeons and a long learning process&#46; Modification of the technique to more closely resemble the surgical procedure in humans is possible&#44; thus facilitating interpretation and allowing more reliable extrapolation to humans&#46;</p>"
      ]
      "es" => array:1 [
        "resumen" => "<span class="elsevierStyleSectionTitle">Objetivos</span><p class="elsevierStyleSimplePara elsevierViewall">Desarrollar el modelo experimental de trasplante pulmonar &#40;TP&#41; con <span class="elsevierStyleItalic">cuffs</span> &#40;t&#233;cnica de manguito&#41;&#44; inicialmente descrito por Mizuta&#44; como medio de estudio del TP en nuestro medio incorporando un nuevo protocolo anest&#233;sico r&#225;pido&#44; seguro y reversible&#46;</p> <span class="elsevierStyleSectionTitle">Material Y M&#233;todo</span><p class="elsevierStyleSimplePara elsevierViewall">Se han utilizado ratas Sprague-Dawley &#40;n &#61; 88&#41; para el desarrollo del modelo experimental y se han logrado realizar 30 TP izquierdos &#40;n &#61; 60&#41;&#46; En el animal donante se han efectuado una esternotom&#237;a media y extracci&#243;n del bloque cardiopulmonar con disecci&#243;n del pulm&#243;n izquierdo y colocaci&#243;n de los <span class="elsevierStyleItalic">cuffs</span> &#40;cat&#233;teres de venopunci&#243;n cortados en cilindros de 3 mm&#41;&#46; En el receptor&#44; a trav&#233;s de una toracotom&#237;a lateral&#44; se ha llevado a cabo el implante del pulm&#243;n izquierdo mediante las anastomosis con <span class="elsevierStyleItalic">cuffs&#46;</span> Se han valorado el tiempo quir&#250;rgico y las complicaciones postoperatorias&#44; as&#237; como la presencia de signos de lesi&#243;n de isquemia-reperfusi&#243;n y rechazo agudo en el pulm&#243;n trasplantado&#46;</p> <span class="elsevierStyleSectionTitle">Resultados</span><p class="elsevierStyleSimplePara elsevierViewall">Durante el desarrollo del modelo experimental rechazamos a 8 animales en la extracci&#243;n&#46; Tras com-pletarla satisfactoriamente&#44; no se pudo realizar el implante en 10 ratas por problemas t&#233;cnicos&#46; De los animales trasplantados&#44; 4 fallecieron en las primeras 24 h y 26 ratas sobrevivieron hasta las 48 h&#44; momento en que fueron sacrificadas&#46; Hallamos un buen estado de las anastomosis y signos de lesi&#243;n de isquemia-reperfusi&#243;n y rechazo agudo en el par&#233;nquima pulmonar del pulm&#243;n trasplantado&#46;</p> <span class="elsevierStyleSectionTitle">Conclusiones</span><p class="elsevierStyleSimplePara elsevierViewall">El TP con <span class="elsevierStyleItalic">cuffs</span> en ratas es un modelo v&#225;lido&#44; fiable&#44; reproducible y econ&#243;mico para el estudio del TP y de los fen&#243;menos de isquemia-reperfusi&#243;n y rechazo&#46; La t&#233;cnica quir&#250;rgica es compleja&#44; tiene una larga curva de aprendizaje y requiere personal con experiencia quir&#250;rgica&#46; Adem&#225;s&#44; es subsidiaria de modificaciones que hagan que se asemeje m&#225;s a la t&#233;cnica quir&#250;rgica utilizada en cl&#237;nica humana para interpretar mejor y poder extrapolar los resultados al humano con m&#225;s fiabilidad&#46;</p>"
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:1 [
        "nota" => "<p class="elsevierStyleNotepara">This project was funded by the Health Research Fund &#40;FIS&#41;&#46;</p>"
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "REFERENCES"
      "seccion" => array:1 [
        0 => array:1 [
          "bibliografiaReferencia" => array:14 [
            0 => array:3 [
              "identificador" => "bib1"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Trasplante pulmonar&#46; Normativa SEPAR"
                      "autores" => array:1 [
                        0 => array:2 [
                          "colaboracion" => "Grupo de Trabajo de Trasplante Pulmonar SEPAR"
                          "etal" => false
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Arch Bronconeumol"
                        "fecha" => "2001"
                        "volumen" => "37"
                        "paginaInicial" => "307"
                        "paginaFinal" => "315"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11562316"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib2"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "V Demikov"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:4 [
                        "titulo" => "Experimental transplantation of vital organ"
                        "fecha" => "1962"
                        "editorial" => "Consultants Bureau Enterprises&#44; Inc&#46;"
                        "editorialLocalizacion" => "New York"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib3"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung allografts in dogs treated with methotrexate and antilymphocyte serum"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "DA Blumenstock"
                            1 => "HP Otte"
                            2 => "OV Grosjean"
                            3 => "MA Mulder"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1968"
                        "volumen" => "6"
                        "paginaInicial" => "33"
                        "paginaFinal" => "39"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/4394021"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib4"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung transplantation in the rat"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "PJ Asimacopoulos"
                            1 => "FAS Molokhia"
                            2 => "CAS Pegg"
                            3 => "JC Norman"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Transplant Proc"
                        "fecha" => "1971"
                        "volumen" => "3"
                        "paginaInicial" => "583"
                        "paginaFinal" => "585"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/4937944"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib5"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung transplantation in the rat&#58; I&#46; Technique and survival"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "KW Marck"
                            1 => "CR Wildevuur"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1982"
                        "volumen" => "34"
                        "paginaInicial" => "74"
                        "paginaFinal" => "80"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7046662"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib6"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Simplified rat lung transplantation using a cuff technique"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "T Mizuta"
                            1 => "A Kawaguchi"
                            2 => "K Nakahara"
                            3 => "Y Kawashima"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Thorac Cardiovasc Surg"
                        "fecha" => "1989"
                        "volumen" => "97"
                        "paginaInicial" => "578"
                        "paginaFinal" => "581"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2648080"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib7"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Improved outcome of rat lung transplantation with modification of the non suture external cuff technique"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A Reis"
                            1 => "A Giaid"
                            2 => "C Serrick"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "1995"
                        "volumen" => "14"
                        "paginaInicial" => "274"
                        "paginaFinal" => "279"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7779846"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib8"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A simplified model for en bloc double lung xeno-transplantation from hamster to rat"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "M de Perrot"
                            1 => "S Keshavjee"
                            2 => "T Tabata"
                            3 => "M Liu"
                            4 => "GP Downey"
                            5 => "TK Waddell"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "2002"
                        "volumen" => "21"
                        "paginaInicial" => "286"
                        "paginaFinal" => "289"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11834358"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib9"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Nitroglycerin alters alveolar type II cell ultrastructure after ischemia and reperfusion"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "A Fehrenbach"
                            1 => "T Wittwer"
                            2 => "D Meyer"
                            3 => "S von Vietinghoff"
                            4 => "M Viehover"
                            5 => "H Fehrenbach"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "2001"
                        "volumen" => "20"
                        "paginaInicial" => "876"
                        "paginaFinal" => "888"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib10"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of a single injection of high-dose FK506 on lung transplantation in rats"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "Y Sano"
                            1 => "S Maruyama"
                            2 => "M Aoe"
                            3 => "H Date"
                            4 => "N Shimizu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Surg Today"
                        "fecha" => "1996"
                        "volumen" => "26"
                        "paginaInicial" => "999"
                        "paginaFinal" => "1005"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9017963"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib11"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of ischemic injury on subsequent rat lung allograft rejection"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "T Shiraishi"
                            1 => "T Mizuta"
                            2 => "SR DeMeester"
                            3 => "JH Ritter"
                            4 => "PE Swanson"
                            5 => "MR Wick"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1995"
                        "volumen" => "60"
                        "paginaInicial" => "947"
                        "paginaFinal" => "951"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7575000"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib12"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ischemia-reperfusion-induced lung injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "M de Perrot"
                            1 => "M Liu"
                            2 => "T Waddell"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Am Resp J Crit Care Med"
                        "fecha" => "2003"
                        "volumen" => "167"
                        "paginaInicial" => "490"
                        "paginaFinal" => "511"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib13"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Improved distribution of pulmonary flush solution to the traqueobronchial wall in pulmonary transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A Varela"
                            1 => "CG Montero"
                            2 => "M C&#243;rdoba"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur Surg Res"
                        "fecha" => "1997"
                        "volumen" => "29"
                        "paginaInicial" => "1"
                        "paginaFinal" => "4"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9286322"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib14"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Optimal inflation volume for hypothermic preservation of rat lungs"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "KN de Campos"
                            1 => "S Keshajvee"
                            2 => "M Liu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "1998"
                        "volumen" => "17"
                        "paginaInicial" => "599"
                        "paginaFinal" => "609"
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/15792129/0000004000000010/v1_201305141820/S1579212906603522/v1_201305141820/en/main.assets"
  "Apartado" => null
  "PDF" => "https://static.elsevier.es/multimedia/15792129/0000004000000010/v1_201305141820/S1579212906603522/v1_201305141820/en/main.pdf?idApp=UINPBA00003Z&text.app=https://archbronconeumol.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212906603522?idApp=UINPBA00003Z"
]
Share
Journal Information
Vol. 40. Issue 10.
Pages 438-442 (October 2004)
Share
Share
Download PDF
More article options
Vol. 40. Issue 10.
Pages 438-442 (October 2004)
Original Articles
Full text access
Lung Transplantation in Rats: a Viable Experimental Model
Visits
4638
N. Santana Rodrígueza,
Corresponding author
norbesr@terra.es

Correspondence: Dr. N. Santana Rodríguez. Unidad de Cirugía Torácica. Clínica San Roque. C/ Juan E. Doreste, 11. 35001 Las Palmas de Gran Canaria. España
, J.L. Martín Barrasab, A. López Garcíac, P. Rodríguez Suárezb, M. Ponce Gonzálezb, J. Freixinet Gilartb
a Unidad de Cirugía Torácica, Clínica San Roque, Las Palmas de Gran Canaria, Spain
b Unidad de Cirugía Torácica, Unidad de Investigación, Hospital Universitario Dr. Negrín, Las Palmas de Gran Canaria, Spain
c Servicio de Anatomía Patológica, Hospital Universitario Puerta de Hierro, Madrid, Spain
This item has received
Article information
Objectives

TO incorporate a new fast, safe, and reversible anesthetic procedure into the experimental model of lung transplantation (LT) using a cuff technique originally described by Mizuta.

Material and Method

Eighty-eight Sprague-Dawley rats were used in the experimental model. Thirty left LTs were performed, using 60 rats. The donor heart-lung block was excised by median sternotomy with dissection of the left lung and cuffs (intravenous catheters cut into 3-mm sections) were put in place. The left lung was implanted in the recipient by lateral thoracotomy using the cuffs for anastomoses. The duration of surgery and postoperative complications were recorded. Also noted were signs of ischemia-reperfusion injury, and acute rejection of the transplanted lung.

Results

We discarded lungs excised from 8 animals when developing the experimental model. Transplants could not be completed in 10 rats due to technical problems, despite satisfactory excision. Of the rats who received a transplant, 4 died in the first 24 hours and 26 survived to 48 hours. They were then killed and examined. The state of the anastomoses was good and signs of ischemia-reperfusion injury, as well as acute rejection were observed in the parenchyma of the transplanted lung.

Conclusions

LT with cuffs in rats is a valid, reliable, reproducible, and cheap model for studying ischemia-reperfusion injury and rejection in LT. The surgical technique is complex, requiring experienced surgeons and a long learning process. Modification of the technique to more closely resemble the surgical procedure in humans is possible, thus facilitating interpretation and allowing more reliable extrapolation to humans.

Key Words:
Lung transplant
Experimental surgery
Experimental transplant
Objetivos

Desarrollar el modelo experimental de trasplante pulmonar (TP) con cuffs (técnica de manguito), inicialmente descrito por Mizuta, como medio de estudio del TP en nuestro medio incorporando un nuevo protocolo anestésico rápido, seguro y reversible.

Material Y Método

Se han utilizado ratas Sprague-Dawley (n = 88) para el desarrollo del modelo experimental y se han logrado realizar 30 TP izquierdos (n = 60). En el animal donante se han efectuado una esternotomía media y extracción del bloque cardiopulmonar con disección del pulmón izquierdo y colocación de los cuffs (catéteres de venopunción cortados en cilindros de 3 mm). En el receptor, a través de una toracotomía lateral, se ha llevado a cabo el implante del pulmón izquierdo mediante las anastomosis con cuffs. Se han valorado el tiempo quirúrgico y las complicaciones postoperatorias, así como la presencia de signos de lesión de isquemia-reperfusión y rechazo agudo en el pulmón trasplantado.

Resultados

Durante el desarrollo del modelo experimental rechazamos a 8 animales en la extracción. Tras com-pletarla satisfactoriamente, no se pudo realizar el implante en 10 ratas por problemas técnicos. De los animales trasplantados, 4 fallecieron en las primeras 24 h y 26 ratas sobrevivieron hasta las 48 h, momento en que fueron sacrificadas. Hallamos un buen estado de las anastomosis y signos de lesión de isquemia-reperfusión y rechazo agudo en el parénquima pulmonar del pulmón trasplantado.

Conclusiones

El TP con cuffs en ratas es un modelo válido, fiable, reproducible y económico para el estudio del TP y de los fenómenos de isquemia-reperfusión y rechazo. La técnica quirúrgica es compleja, tiene una larga curva de aprendizaje y requiere personal con experiencia quirúrgica. Además, es subsidiaria de modificaciones que hagan que se asemeje más a la técnica quirúrgica utilizada en clínica humana para interpretar mejor y poder extrapolar los resultados al humano con más fiabilidad.

Palabras clave:
Trasplante pulmonar
Cirugía experimental
Trasplante experimental
Full text is only aviable in PDF
REFERENCES
[1]
Grupo de Trabajo de Trasplante Pulmonar SEPAR.
Trasplante pulmonar. Normativa SEPAR.
Arch Bronconeumol, 37 (2001), pp. 307-315
[2]
V Demikov.
Experimental transplantation of vital organ, Consultants Bureau Enterprises, Inc., (1962),
[3]
DA Blumenstock, HP Otte, OV Grosjean, MA Mulder.
Lung allografts in dogs treated with methotrexate and antilymphocyte serum.
Ann Thorac Surg, 6 (1968), pp. 33-39
[4]
PJ Asimacopoulos, FAS Molokhia, CAS Pegg, JC Norman.
Lung transplantation in the rat.
Transplant Proc, 3 (1971), pp. 583-585
[5]
KW Marck, CR Wildevuur.
Lung transplantation in the rat: I. Technique and survival.
Ann Thorac Surg, 34 (1982), pp. 74-80
[6]
T Mizuta, A Kawaguchi, K Nakahara, Y Kawashima.
Simplified rat lung transplantation using a cuff technique.
J Thorac Cardiovasc Surg, 97 (1989), pp. 578-581
[7]
A Reis, A Giaid, C Serrick.
Improved outcome of rat lung transplantation with modification of the non suture external cuff technique.
J Heart Lung Transplant, 14 (1995), pp. 274-279
[8]
M de Perrot, S Keshavjee, T Tabata, M Liu, GP Downey, TK Waddell.
A simplified model for en bloc double lung xeno-transplantation from hamster to rat.
J Heart Lung Transplant, 21 (2002), pp. 286-289
[9]
A Fehrenbach, T Wittwer, D Meyer, S von Vietinghoff, M Viehover, H Fehrenbach, et al.
Nitroglycerin alters alveolar type II cell ultrastructure after ischemia and reperfusion.
J Heart Lung Transplant, 20 (2001), pp. 876-888
[10]
Y Sano, S Maruyama, M Aoe, H Date, N Shimizu.
Effect of a single injection of high-dose FK506 on lung transplantation in rats.
Surg Today, 26 (1996), pp. 999-1005
[11]
T Shiraishi, T Mizuta, SR DeMeester, JH Ritter, PE Swanson, MR Wick, et al.
Effect of ischemic injury on subsequent rat lung allograft rejection.
Ann Thorac Surg, 60 (1995), pp. 947-951
[12]
M de Perrot, M Liu, T Waddell.
Ischemia-reperfusion-induced lung injury.
Am Resp J Crit Care Med, 167 (2003), pp. 490-511
[13]
A Varela, CG Montero, M Córdoba.
Improved distribution of pulmonary flush solution to the traqueobronchial wall in pulmonary transplantation.
Eur Surg Res, 29 (1997), pp. 1-4
[14]
KN de Campos, S Keshajvee, M Liu.
Optimal inflation volume for hypothermic preservation of rat lungs.
J Heart Lung Transplant, 17 (1998), pp. 599-609

This project was funded by the Health Research Fund (FIS).

Copyright © 2004. Sociedad Española de Neumología y Cirugía Torácica (SEPAR)
Archivos de Bronconeumología
Article options
Tools

Are you a health professional able to prescribe or dispense drugs?