Expresión de los genes de la interleucina 10 (IL-10) y del factor de necrosis tumoral alfa (TNF-α) en músculos respiratorios y periféricos. Relación con el daño sarcolémico
Interleukin 10 and tumor necrosis factor alpha gene expression in respiratory and peripheral muscle. Relation to sarcolemmal damage
J. Palacioa,
, J.B. Gáldizb, J.J. Bechc, M. Mariñánb, C. Casadevalla, P. Martínezc, J. Geaa
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jgea@imim.es
Correspondencia: Dr. J. Gea. Servicio de Neumología. Hospital del Mar.P.° Marítim, 27. 08003 Barcelona
Correspondencia: Dr. J. Gea. Servicio de Neumología. Hospital del Mar.P.° Marítim, 27. 08003 Barcelona
a Servicio de Neumología. Unidad de Investigación Respiratoria y Ambiental. Hospital del Mar. IMIM. Fisiopatología (CEXS). Universitat Pompeu Fabra. Barcelona
b Servicio de Neumología. Hospital de Cruces. Universidad del País Vasco. Bilbao
c Unidad de Inmunología.Instituto de Biotecnología y Biomedicina. Universitat Autònoma de Barcelona. Barcelona
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Unidad de Investigación Respiratoria y Ambiental. Hospital del Mar. IMIM. Fisiopatología (CEXS). Universitat Pompeu Fabra. Barcelona" "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Servicio de Neumología. Hospital de Cruces. Universidad del País Vasco. Bilbao" "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Unidad de Inmunología.Instituto de Biotecnología y Biomedicina. Universitat Autònoma de Barcelona. Barcelona" "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Correspondencia: Dr. J. Gea. Servicio de Neumología. Hospital del Mar.P.° Marítim, 27. 08003 Barcelona" ] ] ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "Interleukin 10 and tumor necrosis factor alpha gene expression in respiratory and peripheral muscle. Relation to sarcolemmal damage" ] ] "miscelanea" => "Este trabajo ha sido subvencionado por BIOMED (UE) BMH4-CT98-3406, SEPAR 98 y FIS 98" "pdfFichero" => "main.pdf" "tienePdf" => true "PalabrasClave" => array:2 [ "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec190563" "palabras" => array:3 [ 0 => "Músculos respiratorios" 1 => "Músculos periféricos" 2 => "Citocinas" ] ] ] "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec190564" "palabras" => array:3 [ 0 => "Respiratory Muscles" 1 => "Peripheral Muscles" 2 => "Cytokines" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "es" => array:1 [ "resumen" => "<span class="elsevierStyleSectionTitle">Antecedentes</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">El factor de necrosis tumoral alfa (TNF-a) se ha implicado en la pérdida de masa muscular en la enfermedad pulmonar obstructiva crónica (EPOC) y otras enfermedades consumtivas. Su producción estaría en relación con la inflamación derivada de la propia enfermedad pulmonar o el hábito tabáquico, llegando al músculo por el torrente sanguíneo. Sin embargo, también se ha sugerido que el TNF-α podría expresarse directamente en el tejido muscular. Sea cual fuere el lugar de producción, la relación del TNF-α con el daño muscular subsiguiente no es clara</p> <span class="elsevierStyleSectionTitle">Objetivo</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Hemos estudiado la expresión del TNF-α y una interleucina inhibidora de su producción (IL-10) en los músculos respiratorios principales y en un músculo periférico del perro</p> <span class="elsevierStyleSectionTitle">Métodos</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Se han incluido nueve animales machos, adultos jóvenes de raza Beagle. En todos ellos se tomaron biopsias del diafragma (Df), del intercostal externo (IExt) y de un músculo de las extremidades (vasto interno del cuádriceps o VInt). Se analizó la expresión de los genes de TNF-α e IL-10, a través de la determinación de sus ARNm corres-pondientes, utilizando la transcripción reversa y reacción en cadena de la polimerasa (RT-PCR). También se evaluó el daño sarcolémico mediante detección de fibronectina intra-celular (inmunohistoquímica)</p> <span class="elsevierStyleSectionTitle">Resultados</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Los valores de expresión de ambas citocinas evidenciaron una amplia dispersión interindividual. Por un lado, la expresión de TNF-α fue mínima en Df y IExt (respectivamente, 0,02 ± 0,03 y 0,05 ± 0,06 u.a.), con valores relativamente elevados en VInt (0,14 ± 0,08 u.a.). A su vez, la IL-10 evidenciaba valores bajos en DF (0,6 ± 0,5 u.a.), que eran algo superiores en IExt (2,7 ± 1,9 u.a.; p < 0,01) y VInt (1,6 ± 1,7 u.a.). El daño sarcolémico era mínimo en los tres músculos y se relacionaba con la expresión de TNF-α en el músculo periférico (r<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0,682; p < 0,05)</p> <span class="elsevierStyleSectionTitle">Conclusiones</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">a) TNF-α e IL-10 parecen expresarse constitutivamente en el músculo esquelético canino; b) los valores basales de expresión del TNF-α son menores en los músculos repiratorios que en los de las extremidades mos-trando relación en estos últimos con el daño de membrana</p>" ] "en" => array:1 [ "resumen" => "<span class="elsevierStyleSectionTitle">Background</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Tumor necrosis factor alpha (TNF-α) has been implicated in loss of muscle mass in chronic obstructive pulmonary disease and other consumptive processes. TNF-α production would be related to inflammation arising from pulmonary disease itself or, alternatively, from smoking, and would be carried to the muscle through the blood stream. However, it has also been suggested that TNF-α may be expressed directly in muscle tissue. Regardless the site of production of TNF-α, its relation to subsequent muscle damage is unclear</p> <span class="elsevierStyleSectionTitle">Objective</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">We studied the expression of TNF-α and an interleukin inhibitor of its production (IL-10) in the main respiratory muscles and a peripheral muscle in the dog</p> <span class="elsevierStyleSectionTitle">Method</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Nine young, male Beagle dogs were included. From all animals we obtained a biopsy of the diaphragm (Dph) and external intercostal (ExtI) muscles and a leg muscle (internal vastus of the quadriceps, IntV). TNF-α and IL-10 gene expressions were measured through the analysis of mes-senger RNA levels, using reverse transcription and polymera-se chain reaction. We also assessed sarcolemmal damage using intracellular fibronectin detection (immunohistochemistry)</p> <span class="elsevierStyleSectionTitle">Results</span><p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">The expression of both cytokines showed wide in-terindividual variability. On the one hand, TNF-α (was very low in Dph and ExtI (0.02 ± 0.03 and 0.05 ± 0.06 a.u., respec-tively), but relatively high in the IntV (0.14 ± 0.08 a.u.). IL-10 expression, on the other hand was low in the Dph (0.06 ± 0.05 a.u.) and slightly higher in the ExtI (2.7 ± 1.9 a.u., p < 0.01) and IntV (1.6 ± 1.7 a.u.). Sarcolemmal damage was minimal in all three muscles and was related to TNF-α expression in the peripheral muscle (r<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.682, p < 0.05)</p> <span class="elsevierStyleSectionTitle">Conclusions</span><p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">1) TNF-α and IL-10 appear to be constitutively expressed within the skeletal muscle in dogs. 2) Basal TNF-α expression is lower in respiratory muscles than in peripheral muscles. 3) The expression in the latter is related to membrane damage</p>" ] ] "bibliografia" => array:2 [ "titulo" => "Bibliografía" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:29 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Responses to exercise in lung diseases. En: Wipp Roca J, editors. 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| Year/Month | Html | Total | |
|---|---|---|---|
| 2024 November | 5 | 10 | 15 |
| 2024 October | 46 | 71 | 117 |
| 2024 September | 44 | 50 | 94 |
| 2024 August | 67 | 55 | 122 |
| 2024 July | 29 | 34 | 63 |
| 2024 June | 47 | 41 | 88 |
| 2024 May | 38 | 43 | 81 |
| 2024 April | 33 | 37 | 70 |
| 2024 March | 25 | 28 | 53 |
| 2024 February | 24 | 54 | 78 |
| 2024 January | 0 | 14 | 14 |
| 2023 December | 0 | 4 | 4 |
| 2023 November | 0 | 14 | 14 |
| 2023 October | 0 | 3 | 3 |
| 2023 September | 0 | 3 | 3 |
| 2023 August | 0 | 1 | 1 |
| 2023 July | 0 | 6 | 6 |
| 2023 June | 0 | 13 | 13 |
| 2023 April | 0 | 2 | 2 |
| 2023 March | 5 | 11 | 16 |
| 2023 February | 27 | 29 | 56 |
| 2023 January | 26 | 32 | 58 |
| 2022 December | 38 | 45 | 83 |
| 2022 November | 39 | 34 | 73 |
| 2022 October | 27 | 43 | 70 |
| 2022 September | 27 | 34 | 61 |
| 2022 August | 37 | 58 | 95 |
| 2022 July | 33 | 35 | 68 |
| 2022 June | 22 | 43 | 65 |
| 2022 May | 40 | 29 | 69 |
| 2022 April | 36 | 43 | 79 |
| 2022 March | 34 | 35 | 69 |
| 2022 February | 34 | 28 | 62 |
| 2022 January | 28 | 26 | 54 |
| 2021 December | 31 | 56 | 87 |
| 2021 November | 28 | 36 | 64 |
| 2021 October | 21 | 38 | 59 |
| 2021 September | 24 | 42 | 66 |
| 2021 August | 18 | 36 | 54 |
| 2021 July | 20 | 23 | 43 |
| 2021 June | 28 | 43 | 71 |
| 2021 May | 43 | 40 | 83 |
| 2021 April | 27 | 92 | 119 |
| 2021 March | 35 | 32 | 67 |
| 2021 February | 42 | 13 | 55 |
| 2021 January | 41 | 27 | 68 |
| 2020 December | 37 | 21 | 58 |
| 2020 November | 24 | 9 | 33 |
| 2020 October | 27 | 9 | 36 |
| 2020 September | 15 | 2 | 17 |
| 2020 August | 26 | 13 | 39 |
| 2020 July | 49 | 20 | 69 |
| 2020 June | 23 | 9 | 32 |
| 2020 May | 24 | 16 | 40 |
| 2020 April | 31 | 15 | 46 |
| 2020 March | 31 | 17 | 48 |
| 2020 February | 19 | 25 | 44 |
| 2020 January | 39 | 32 | 71 |
| 2019 December | 50 | 29 | 79 |
| 2019 November | 9 | 12 | 21 |
| 2019 October | 23 | 15 | 38 |
| 2019 September | 15 | 27 | 42 |
| 2019 August | 14 | 14 | 28 |
| 2019 July | 16 | 26 | 42 |
| 2019 June | 16 | 21 | 37 |
| 2019 May | 35 | 59 | 94 |
| 2019 April | 25 | 64 | 89 |
| 2019 March | 14 | 27 | 41 |
| 2019 February | 14 | 17 | 31 |
| 2019 January | 14 | 22 | 36 |
| 2018 December | 21 | 31 | 52 |
| 2018 November | 29 | 42 | 71 |
| 2018 October | 40 | 52 | 92 |
| 2018 September | 29 | 47 | 76 |
| 2018 May | 14 | 1 | 15 |
| 2018 April | 7 | 15 | 22 |
| 2018 March | 20 | 8 | 28 |
| 2018 February | 9 | 9 | 18 |
| 2018 January | 11 | 21 | 32 |
| 2017 December | 14 | 23 | 37 |
| 2017 November | 10 | 16 | 26 |
| 2017 October | 13 | 32 | 45 |
| 2017 September | 6 | 18 | 24 |
| 2017 August | 13 | 20 | 33 |
| 2017 July | 16 | 21 | 37 |
| 2017 June | 16 | 15 | 31 |
| 2017 May | 11 | 29 | 40 |
| 2017 April | 9 | 38 | 47 |
| 2017 March | 11 | 18 | 29 |
| 2017 February | 7 | 25 | 32 |
| 2017 January | 4 | 10 | 14 |
| 2016 December | 37 | 24 | 61 |
| 2016 November | 34 | 26 | 60 |
| 2016 October | 34 | 31 | 65 |
| 2016 September | 47 | 22 | 69 |
| 2016 August | 31 | 8 | 39 |
| 2016 July | 37 | 9 | 46 |
| 2016 June | 33 | 12 | 45 |
| 2016 May | 27 | 5 | 32 |
| 2016 April | 15 | 1 | 16 |
| 2016 March | 27 | 4 | 31 |
| 2016 February | 20 | 3 | 23 |
| 2016 January | 42 | 10 | 52 |
| 2015 December | 30 | 9 | 39 |
| 2015 November | 39 | 4 | 43 |
| 2015 October | 100 | 1 | 101 |
| 2015 September | 80 | 0 | 80 |
| 2015 August | 83 | 0 | 83 |
| 2015 July | 77 | 0 | 77 |
| 2015 June | 79 | 0 | 79 |
| 2015 May | 105 | 0 | 105 |
| 2015 April | 66 | 0 | 66 |
| 2015 March | 74 | 0 | 74 |
| 2015 February | 54 | 0 | 54 |
| 2015 January | 73 | 0 | 73 |
| 2014 December | 48 | 0 | 48 |
| 2014 November | 75 | 0 | 75 |
| 2014 October | 75 | 0 | 75 |
| 2014 September | 60 | 0 | 60 |
| 2014 August | 53 | 0 | 53 |
| 2014 July | 47 | 0 | 47 |
| 2014 June | 65 | 0 | 65 |
| 2014 May | 96 | 0 | 96 |
| 2014 April | 62 | 0 | 62 |
| 2014 March | 91 | 0 | 91 |
| 2014 February | 68 | 0 | 68 |
| 2014 January | 59 | 0 | 59 |
| 2013 December | 51 | 0 | 51 |
| 2013 November | 79 | 0 | 79 |
| 2013 October | 88 | 0 | 88 |
| 2013 September | 58 | 0 | 58 |
| 2013 August | 65 | 0 | 65 |
| 2013 July | 66 | 0 | 66 |
| 2013 June | 50 | 0 | 50 |
| 2013 May | 41 | 0 | 41 |
| 2013 April | 26 | 0 | 26 |
| 2013 March | 12 | 0 | 12 |
| 2000 January | 1977 | 0 | 1977 |
Show all
