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Vol. 46. Issue 5.
Pages 215-222 (May 2010)
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Vol. 46. Issue 5.
Pages 215-222 (May 2010)
Original Article
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Effect of Inhaled Fluticasone on Lung Inflammation Administered During and After Guinea Pig Sensitization
Efecto de la fluticasona inhalada sobre la inflamación pulmonar administrada durante y después de la sensibilización de cobayas
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Mohammad Hossein Boskabadya,
Corresponding author
, Mohamad Javad Eslamizadea, Abas Tabatabaeib, Habib Nematia, Fariba Mansouric
a Department of Physiology and Pharmacological Research Centre of Medical Plants, Mashhad, Iran
b Department of Pathology, Ghaem Medical Centre, Mashhad University of Medical Sciences, Mashhad, Iran
c Department of Medicine, Kermanshah University of Medical Sciences Kermanshah, Iran
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Abstract
Objective

The effect of an inhaled corticosteroid, fluticasone propionate (FP) lung inflammation of sensitized guinea pig was examined.

Material and methods

Four groups of guinea pigs (n = 8) were sensitized (S) with ovalbumin (OA). Control group was given similar solutions without OA. One S group was treated with inhaled 250#mg inhaled FP twice/day during, other group after sensitization for 18 days and two groups were treated with placebo, one during, and the other after sensitization. One day after the last treatment, tracheal responses of all animal groups to methacholine and OA were examined. Total and differential white blood cell (WBC) counts of lung lavage and lung pathology were also examined.

Results

Tracheal responsiveness to both methacholine and OA and WBC of both placebo groups were significantly higher than those of control group (P < 0.001 for all cases). The lungs of placebo groups showed variable pathological changes (non significant to P < 0.001) compared to control group. Tracheal responsiveness in two treated groups with FP to both methacholine and OA were significantly decreased compared to placebo groups (P < 0.01 to P < 0.001). Treatment with FP leads to improvement in total (P < 0.001) and differential WBC counts (non significant to P < 0.001) as well as mucosal detachment (P < 0.001), but not other pathological changes.

Conclusions

These results showed a protective effect of FP on tracheal responsiveness and lung inflammation. In addition, this study showed that treatment with inhaled fluticasone propionate, during sensitization (development of inflammation and pathological changes) was more effective than after sensitization (establishment of inflammation and pathological changes).

Keywords:
Fluticasone propionate
Asthma
Tracheal responsiveness
Sensitization
Inflammation
Resumen
Objetivo

Se examinó el efecto de un corticoesteroide inhalado, el propionato de fluticasona (PF), sobre la inflamación pulmonar de cobayas sensibilizados.

Material y métodos

Se sensibilizó con ovoalbúmina (OA) a 4 grupos de cobayas (n = 8). El grupo de control recibió soluciones similares sin OA. Durante un periodo de 18 días, un grupo sensibilizado fue tratado con 250 μg de PF inhalado 2 veces al día durante la sensibilización; el otro grupo después de ella y los otros 2 grupos fueron tratados con placebo, uno durante la sensibilización y otro después de ella. A las 24 h del último tratamiento, se examinaron las respuestas traqueales de todos los grupos de animales a metacolina y OA. También se examinaron el recuento total de leucocitos y la fórmula leucocitaria del líquido de lavado pulmonar y la anatomía patológica pulmonar.

Resultados

En ambos grupos placebo la reactividad traqueal tanto a metacolina como a OA y el recuento de leucocitos fueron significativamente mayores que los del grupo de control (p < 0,001 para todos los casos). En los grupos placebo se demostraron cambios anatomopatológicos variables de los pulmones (no significativos hasta p < 0,001), comparado con los grupos de control. En los 2 grupos tratados con PF la reactividad traqueal tanto a metacolina como a OA disminuyó significativamente comparado con los grupos placebo (p < 0,01 hasta p < 0,001). El tratamiento con PF da lugar a una mejora del recuento de leucocitos (p < 0,001) y de la fórmula leucocitaria (no significativo p < 0,001) al igual que del desprendimiento mucoso (p < 0,001), pero no otros cambios anatomopatológicos.

Conclusiones

Estos resultados demuestran un efecto protector del PF sobre la reactividad traqueal y la inflamación pulmonar. Además, el presente estudio demostró que el tratamiento con propionato de fluticasona inhalado durante la sensibilización (desarrollo de inflamación y cambios anatomopatológicos) fue más eficaz que después de ella (establecimiento de inflamación y cambios anatomopatológicos).

Palabras clave:
Propionato de fluticasona
Asma
Reactividad traqueal
Sensibilización
Inflamación
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