array:23 [
  "pii" => "S1579212907600883"
  "issn" => "15792129"
  "doi" => "10.1016/S1579-2129(07)60088-3"
  "estado" => "S300"
  "fechaPublicacion" => "2007-01-01"
  "aid" => "60088"
  "copyright" => "Sociedad Española de Neumología y Cirugía Torácica (SEPAR)"
  "copyrightAnyo" => "2007"
  "documento" => "article"
  "crossmark" => 0
  "subdocumento" => "fla"
  "cita" => "Arch Bronconeumol. 2007;43:373-7"
  "abierto" => array:3 [
    "ES" => false
    "ES2" => false
    "LATM" => false
  ]
  "gratuito" => false
  "lecturas" => array:2 [
    "total" => 2480
    "formatos" => array:3 [
      "EPUB" => 90
      "HTML" => 1830
      "PDF" => 560
    ]
  ]
  "itemSiguiente" => array:18 [
    "pii" => "S1579212907600895"
    "issn" => "15792129"
    "doi" => "10.1016/S1579-2129(07)60089-5"
    "estado" => "S300"
    "fechaPublicacion" => "2007-01-01"
    "aid" => "60089"
    "copyright" => "Sociedad Española de Neumología y Cirugía Torácica (SEPAR)"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Arch Bronconeumol. 2007;43:378-85"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 2070
      "formatos" => array:3 [
        "EPUB" => 73
        "HTML" => 1397
        "PDF" => 600
      ]
    ]
    "en" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
      "titulo" => "Spanish Medical Center Collaboration on Smoking Research From 1999 Through 2003 According to the Science Citation Index"
      "tienePdf" => "en"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "378"
          "paginaFinal" => "385"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Santiago Jos&#233; Villanueva Serrano, Jos&#233; Ignacio de Granda Orive, Rafael Aleixandre Benavent, Francisco Garc&#237;a R&#237;o, Juan Carlos Valderrama Zuri&#225;n, Adolfo Alonso Arroyo"
          "autores" => array:6 [
            0 => array:2 [
              "nombre" => "Santiago Jos&#233; Villanueva"
              "apellidos" => "Serrano"
            ]
            1 => array:2 [
              "nombre" => "Jos&#233; Ignacio"
              "apellidos" => "de Granda Orive"
            ]
            2 => array:2 [
              "nombre" => "Rafael Aleixandre"
              "apellidos" => "Benavent"
            ]
            3 => array:2 [
              "nombre" => "Francisco Garc&#237;a"
              "apellidos" => "R&#237;o"
            ]
            4 => array:2 [
              "nombre" => "Juan Carlos Valderrama"
              "apellidos" => "Zuri&#225;n"
            ]
            5 => array:2 [
              "nombre" => "Adolfo Alonso"
              "apellidos" => "Arroyo"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907600895?idApp=UINPBA00003Z"
    "url" => "/15792129/0000004300000007/v1_201305150322/S1579212907600895/v1_201305150322/en/main.assets"
  ]
  "itemAnterior" => array:18 [
    "pii" => "S1579212907600871"
    "issn" => "15792129"
    "doi" => "10.1016/S1579-2129(07)60087-1"
    "estado" => "S300"
    "fechaPublicacion" => "2007-01-01"
    "aid" => "60087"
    "copyright" => "Sociedad Espa&#241;ola de Neumolog&#237;a y Cirug&#237;a Tor&#225;cica &#40;SEPAR&#41;"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Arch Bronconeumol. 2007;43:366-72"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 3229
      "formatos" => array:3 [
        "EPUB" => 85
        "HTML" => 2296
        "PDF" => 848
      ]
    ]
    "en" => array:10 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original Articles</span>"
      "titulo" => "Analysis of Energy Expenditure in Adults With Cystic Fibrosis&#58; Comparison of Indirect Calorimetry and Prediction Equations"
      "tienePdf" => "en"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "366"
          "paginaFinal" => "372"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Casilda Olveira Fuster, Gabriel Olveira Fuster, Antonio Dorado Galindo, Alicia Padilla Galo, Julio Merino Verdugo, Francisco Miralles Lozano"
          "autores" => array:6 [
            0 => array:2 [
              "nombre" => "Casilda Olveira"
              "apellidos" => "Fuster"
            ]
            1 => array:2 [
              "nombre" => "Gabriel Olveira"
              "apellidos" => "Fuster"
            ]
            2 => array:2 [
              "nombre" => "Antonio Dorado"
              "apellidos" => "Galindo"
            ]
            3 => array:2 [
              "nombre" => "Alicia Padilla"
              "apellidos" => "Galo"
            ]
            4 => array:2 [
              "nombre" => "Julio Merino"
              "apellidos" => "Verdugo"
            ]
            5 => array:2 [
              "nombre" => "Francisco Miralles"
              "apellidos" => "Lozano"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907600871?idApp=UINPBA00003Z"
    "url" => "/15792129/0000004300000007/v1_201305150322/S1579212907600871/v1_201305150322/en/main.assets"
  ]
  "en" => array:14 [
    "idiomaDefecto" => true
    "titulo" => "Assessment of Ischemia&#8211;Reperfusion Injury and Early Acute Rejection in Experimental Lung Transplantation After Prolonged Ischemia"
    "tieneTextoCompleto" => 0
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "373"
        "paginaFinal" => "377"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "Norberto Santana Rodr&#237;guez, Jos&#233; Luis Mart&#237;n Barrasa, Miguel &#193;ngel Ponce Gonz&#225;lez, Ana L&#243;pez Garc&#237;a, Jos&#233; Antonio Ruiz Caballero, Antonio Torres Garc&#237;a, Jorge Freixinet Gilart"
        "autores" => array:7 [
          0 => array:4 [
            "nombre" => "Norberto Santana"
            "apellidos" => "Rodr&#237;guez"
            "email" => array:1 [
              0 => "norbesr&#64;terra&#46;es"
            ]
            "referencia" => array:3 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
              2 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor1"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "Jos&#233; Luis Mart&#237;n"
            "apellidos" => "Barrasa"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "Miguel &#193;ngel Ponce"
            "apellidos" => "Gonz&#225;lez"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff3"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "Ana L&#243;pez"
            "apellidos" => "Garc&#237;a"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">d</span>"
                "identificador" => "aff4"
              ]
            ]
          ]
          4 => array:3 [
            "nombre" => "Jos&#233; Antonio Ruiz"
            "apellidos" => "Caballero"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">e</span>"
                "identificador" => "aff5"
              ]
            ]
          ]
          5 => array:3 [
            "nombre" => "Antonio Torres"
            "apellidos" => "Garc&#237;a"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">f</span>"
                "identificador" => "aff6"
              ]
            ]
          ]
          6 => array:3 [
            "nombre" => "Jorge Freixinet"
            "apellidos" => "Gilart"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:6 [
          0 => array:3 [
            "entidad" => "Unidad de Cirug&#237;a Tor&#225;cica&#44; Hospital Universitario Insular de Gran Canaria&#44; Las Palmas de Gran Canaria Dr&#46; Negr&#237;n&#44; Las Palmas&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "aff1"
          ]
          1 => array:3 [
            "entidad" => "Unidad de Cirug&#237;a Experimental and Unidad de Investigaci&#243;n&#44; Hospital Universitario de Gran Canaria Dr&#46; Negr&#237;n&#44; Las Palmas de Gran Canaria&#44; Las Palmas&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "aff2"
          ]
          2 => array:3 [
            "entidad" => "Unidad de Hospitalizaci&#243;n Domiciliaria&#44; Hospital Universitario de Gran Canaria Dr&#46; Negr&#237;n&#44; Las Palmas de Gran Canaria&#44; Las Palmas&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">c</span>"
            "identificador" => "aff3"
          ]
          3 => array:3 [
            "entidad" => "Servicio de Anatom&#237;a Patol&#243;gica&#44; Hospital Universitario Cl&#237;nica Puerta de Hierro&#44; Madrid&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">d</span>"
            "identificador" => "aff4"
          ]
          4 => array:3 [
            "entidad" => "Departamento de Ciencias Cl&#237;nicas&#44; Universidad de Las Palmas de Gran Canaria&#44; Las Palmas&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">e</span>"
            "identificador" => "aff5"
          ]
          5 => array:3 [
            "entidad" => "Unidad de Cirug&#237;a Tor&#225;cica and Trasplante Pulmonar de Donante en Asistolia&#44; Hospital Cl&#237;nico San Carlos&#44; Madrid&#44; Spain"
            "etiqueta" => "<span class="elsevierStyleSup">f</span>"
            "identificador" => "aff6"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor1"
            "etiqueta" => "&#42;"
            "correspondencia" => "Correspondence&#58; Dr&#46; N&#46; Santana Rodr&#237;guez&#44; Unidad de Cirug&#237;a Tor&#225;cica&#44; Unidad de Investigaci&#243;n&#44; Hospital Universitario Insular de Gran Canaria&#44; Dr&#46; Negr&#237;n Mar&#237;tima del Sur&#44; s&#47;n&#46; 35010 Las Palmas de Gran Canarias&#46; Las Palmas&#46; Espa&#241;a"
          ]
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2006-04-24"
    "fechaAceptado" => "2006-09-26"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Key words"
          "identificador" => "xpalclavsec154925"
          "palabras" => array:3 [
            0 => "Lung transplantation"
            1 => "Ischemic time"
            2 => "Experimental surgery"
          ]
        ]
      ]
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec154926"
          "palabras" => array:3 [
            0 => "Trasplante pulmonar"
            1 => "Tiempo de isquemia"
            2 => "Cirug&#237;a experimental"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:1 [
        "resumen" => "<span class="elsevierStyleSectionTitle">OBJECTIVE</span><p class="elsevierStyleSimplePara elsevierViewall">To assess ischemia&#8211;reperfusion injury and early acute rejection of the lung subjected to ischemia for 10 hours&#46;</p> <span class="elsevierStyleSectionTitle">MATERIAL AND METHODS</span><p class="elsevierStyleSimplePara elsevierViewall">Fifteen of 30 Sprague&#8211;Dawley rats underwent transplantation of a left lung that had been subjected to ischemic times of 4 &#40;n&#61;5&#41;&#44; 6 &#40;n&#61;5&#41;&#44; or 10 hours &#40;n&#61;5&#41;&#46; The cardiopulmonary block was removed from the donor&#44; the left lung was dissected&#44; and the transplant was carried out using the cuff technique&#46; The cardiopulmonary block was extracted after 48 hours&#46; We assessed postoperative progress&#44; ischemia&#8211;reperfusion injury and acute rejection of the transplanted and contralateral lungs&#46; Statistical probabilities were analyzed using the &#967;<span class="elsevierStyleSup">2</span> and Fisher exact tests&#46;</p> <span class="elsevierStyleSectionTitle">RESULTS</span><p class="elsevierStyleSimplePara elsevierViewall">Clinical course was not worse after an ischemic time of 10 hours &#40;&#61;&#46;711&#41;&#46; No significant differences were observed in histological markers of ischemia&#8211;reperfusion injury and acute rejection or in clinical course in relation to the different ischemic times&#59; nor was clinical course related to the presence or severity of lesions or rejection&#46; Similarly&#44; acute rejection was unrelated to ischemia&#8211;reperfusion injury &#40;&#62;&#46;05&#41;&#46;</p> <span class="elsevierStyleSectionTitle">CONCLUSIONS</span><p class="elsevierStyleSimplePara elsevierViewall">In this study&#44; a prolonged ischemic time of 10 hours was not associated with ischemia&#8211;reperfusion injuries&#44; with more severe acute rejection&#44; or with a worse clinical course&#46; Acute rejection was also unrelated to the presence or severity of ischemia&#8211;reperfusion injury&#46;</p>"
      ]
      "es" => array:1 [
        "resumen" => "<span class="elsevierStyleSectionTitle">OBJETIVO</span><p class="elsevierStyleSimplePara elsevierViewall">Valorar la lesi&#243;n de isquemia-reperfusi&#243;n y el rechazo agudo precoz del pulm&#243;n sometido a un tiempo de isquemia de 10 h&#46;</p> <span class="elsevierStyleSectionTitle">MATERIAL Y M&#201;TODOS</span><p class="elsevierStyleSimplePara elsevierViewall">Se han utilizado 30 ratas Sprague-Dawley&#44; en 15 de las cuales se realiz&#243; un trasplante pulmonar izquierdo con tiempos de isquemia de 4 h &#40;n &#61; 5&#41;&#44; 6 h &#40;n &#61; 5&#41; y 10 h &#40;n &#61; 5&#41;&#46; Del donante se extrajo el bloque cardiopulmonar&#44; se disec&#243; el pulm&#243;n izquierdo y se efectu&#243; el implante con la t&#233;cnica de manguitos <span class="elsevierStyleItalic">&#40;cuffs&#41;</span>&#46; A las 48 h se extrajo el bloque cardiopulmonar&#46; Se valoraron la evoluci&#243;n postoperatoria&#44; la lesi&#243;n de isquemia-reperfusi&#243;n y el rechazo agudo del &#967;<span class="elsevierStyleSup">2</span> pulm&#243;n trasplantado y del contralateral&#46; El an&#225;lisis estad&#237;stico se realiz&#243; con el test de la &#967;<span class="elsevierStyleSup">2</span> y el test exacto de Fisher para el c&#225;lculo de probabilidades&#46;</p> <span class="elsevierStyleSectionTitle">RESULTADOS</span><p class="elsevierStyleSimplePara elsevierViewall">Los animales trasplantados con un tiempo de isquemia de 10 h no tuvieron peor evoluci&#243;n cl&#237;nica &#40;p &#61; 0&#44;711&#41;&#46; No se observaron diferencias significativas entre los par&#225;metros histol&#243;gicos de lesi&#243;n de isquemia-reperfusi&#243;n y de rechazo agudo con los distintos tiempos de isquemia&#44; ni en la evoluci&#243;n cl&#237;nica por la presencia y gravedad de &#233;stos&#46; Tampoco se observ&#243; que el rechazo agudo se relacionara con la lesi&#243;n de isquemia-reperfusi&#243;n &#40;p &#62; 0&#44;05&#41;&#46;</p> <span class="elsevierStyleSectionTitle">CONCLUSIONES</span><p class="elsevierStyleSimplePara elsevierViewall">En nuestro estudio&#44; el tiempo de isquemia pulmonar prolongado de 10 h no se asocia ni a lesiones de isquemia-reperfusi&#243;n y rechazo agudo m&#225;s graves ni a una peor evoluci&#243;n cl&#237;nica&#46; El rechazo agudo no se relaciona con la presencia ni con la gravedad de la lesi&#243;n de isquemiareperfusi&#243;n&#46;</p>"
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:1 [
        "nota" => "<p class="elsevierStyleNotepara">This project was funded by the Spanish Health Research Fund &#40;Fondo de Investigaci&#243;n Sanitaria Espa&#241;ol&#44; or FIS&#41;&#44; the Canary Island Research Foundation &#40;Fundaci&#243;n Canaria de Investigaci&#243;n y Salud&#44; or FUNCIS&#44; project 1104&#41;&#44; the Canary Island Cancer Research Institute &#40;Instituto Canario de Investigaci&#243;n del C&#225;ncer&#44; ICIC&#41; and the Madrid MMA Foundation &#40;Fundaci&#243;n MMA&#41;&#46;</p>"
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "REFERENCES"
      "seccion" => array:1 [
        0 => array:1 [
          "bibliografiaReferencia" => array:15 [
            0 => array:3 [
              "identificador" => "bib1"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary allograft ischemic time&#58; an important predictor of survival after lung transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "GI Snell"
                            1 => "M Rabinov"
                            2 => "A Griffiths"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "1996"
                        "volumen" => "15"
                        "paginaInicial" => "160"
                        "paginaFinal" => "168"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8672519"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib2"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Graft ischemic time and outcome of lung transplantation&#58; a multicenter analysis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "G Thabut"
                            1 => "H Mal"
                            2 => "J Cerrina"
                            3 => "P Dartevelle"
                            4 => "C Dromer"
                            5 => "JF Velly"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.200409-1248OC"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2005"
                        "volumen" => "171"
                        "paginaInicial" => "786"
                        "paginaFinal" => "791"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15665320"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib3"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of lung allograft ischemia duration on post reperfusion graft function and postoperative course"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "M Bund"
                            1 => "H Struber"
                            2 => "J Heine"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1055/s-2007-1010197"
                      "Revista" => array:6 [
                        "tituloSerie" => "Thorac Cardiovasc Surg"
                        "fecha" => "1998"
                        "volumen" => "46"
                        "paginaInicial" => "93"
                        "paginaFinal" => "96"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9618811"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib4"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Impact of graft ischemic time on outcomes after bilateral sequential single-lung transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "T Ueno"
                            1 => "GI Snell"
                            2 => "TJ Williams"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1999"
                        "volumen" => "67"
                        "paginaInicial" => "1577"
                        "paginaFinal" => "1582"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10391258"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib5"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Influence of graft ischemic time and donor age on survival after lung transplantation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "RJ Novick"
                            1 => "LE Benneth"
                            2 => "DM Meyer"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "1999"
                        "volumen" => "18"
                        "paginaInicial" => "425"
                        "paginaFinal" => "431"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10363686"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib6"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Outcome in paired recipients of single lung transplants from the same donor"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "AR Glanville"
                            1 => "D Marshman"
                            2 => "A Keogh"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Heart Lung Transplant"
                        "fecha" => "1995"
                        "volumen" => "14"
                        "paginaInicial" => "878"
                        "paginaFinal" => "882"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8800723"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib7"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Trasplante pulmonar en ratas&#46; Un modelo viable de estudio experimental"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "N Santana Rodr&#237;guez"
                            1 => "L Mart&#237;n Barrasa"
                            2 => "A L&#243;pez Garc&#237;a"
                            3 => "P Rodr&#237;guez Su&#225;rez"
                            4 => "M Ponce Gonz&#225;lez"
                            5 => "J Freixinet Gilart"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Arch Bronconeumol"
                        "fecha" => "2004"
                        "volumen" => "40"
                        "paginaInicial" => "438"
                        "paginaFinal" => "442"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15491534"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib8"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ischemia-reperfusion-induced lung injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "M de Perrot"
                            1 => "M Liu"
                            2 => "T Waddell"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Am Resp J Crit Care Med"
                        "fecha" => "2003"
                        "volumen" => "167"
                        "paginaInicial" => "490"
                        "paginaFinal" => "511"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib9"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung preservation threshold in a compromised septic lung injury model"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S Sasaki"
                            1 => "JD McCully"
                            2 => "JD Palombo"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1995"
                        "volumen" => "60"
                        "paginaInicial" => "958"
                        "paginaFinal" => "963"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7575002"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib10"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prolonged ischemia is associated with more pronounced rejection in the lung allograft"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "C Serrick"
                            1 => "A Giaid"
                            2 => "A Reis"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1997"
                        "volumen" => "63"
                        "paginaInicial" => "202"
                        "paginaFinal" => "208"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8993266"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib11"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung transplantation in the rat&#58; histopathology of left lung iso &#8211; and allografts"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "KW Marck"
                            1 => "J Prop"
                            2 => "CR Wildevuur"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Heart Transplant"
                        "fecha" => "1985"
                        "volumen" => "4"
                        "paginaInicial" => "263"
                        "paginaFinal" => "266"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3916496"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib12"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of ischemic injury on subsequent rat lung allograft rejection"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "T Shiraishi"
                            1 => "T Mizuta"
                            2 => "S DeMeester"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1995"
                        "volumen" => "60"
                        "paginaInicial" => "947"
                        "paginaFinal" => "951"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7575000"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib13"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Right lung transplantation followed by left pneumonectomy in the rat"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "AT Kawaguchi"
                            1 => "T Mizuta"
                            2 => "M Shirai"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur J Cardiothorac Surg"
                        "fecha" => "1996"
                        "volumen" => "10"
                        "paginaInicial" => "1011"
                        "paginaFinal" => "1014"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8971515"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib14"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary preservation studies&#58; effects on endothelial function and pulmonary adenine nucleotides"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "HC Paik"
                            1 => "SC Hoffmann"
                            2 => "TM Egan"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1097/01.TP.0000045685.09601.9E"
                      "Revista" => array:6 [
                        "tituloSerie" => "Transplantation"
                        "fecha" => "2003"
                        "volumen" => "75"
                        "paginaInicial" => "439"
                        "paginaFinal" => "444"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12605106"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib15"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Contralateral lung injury associated with single-lung ischemiareperfusion injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "A Watanabe"
                            1 => "N Kawaharada"
                            2 => "K Kusajima"
                            3 => "S Komatsu"
                            4 => "H Takahashi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Thorac Surg"
                        "fecha" => "1996"
                        "volumen" => "62"
                        "paginaInicial" => "1644"
                        "paginaFinal" => "1649"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8957366"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/15792129/0000004300000007/v1_201305150322/S1579212907600883/v1_201305150322/en/main.assets"
  "Apartado" => null
  "PDF" => "https://static.elsevier.es/multimedia/15792129/0000004300000007/v1_201305150322/S1579212907600883/v1_201305150322/en/main.pdf?idApp=UINPBA00003Z&text.app=https://archbronconeumol.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1579212907600883?idApp=UINPBA00003Z"
]
Share
Journal Information
Vol. 43. Issue 7.
Pages 373-377 (January 2007)
Share
Share
Download PDF
More article options
Vol. 43. Issue 7.
Pages 373-377 (January 2007)
Full text access
Assessment of Ischemia–Reperfusion Injury and Early Acute Rejection in Experimental Lung Transplantation After Prolonged Ischemia
Visits
4458
Norberto Santana Rodrígueza,b,
Corresponding author
norbesr@terra.es

Correspondence: Dr. N. Santana Rodríguez, Unidad de Cirugía Torácica, Unidad de Investigación, Hospital Universitario Insular de Gran Canaria, Dr. Negrín Marítima del Sur, s/n. 35010 Las Palmas de Gran Canarias. Las Palmas. España
, José Luis Martín Barrasab, Miguel Ángel Ponce Gonzálezc, Ana López Garcíad, José Antonio Ruiz Caballeroe, Antonio Torres Garcíaf, Jorge Freixinet Gilarta
a Unidad de Cirugía Torácica, Hospital Universitario Insular de Gran Canaria, Las Palmas de Gran Canaria Dr. Negrín, Las Palmas, Spain
b Unidad de Cirugía Experimental and Unidad de Investigación, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Las Palmas, Spain
c Unidad de Hospitalización Domiciliaria, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Las Palmas, Spain
d Servicio de Anatomía Patológica, Hospital Universitario Clínica Puerta de Hierro, Madrid, Spain
e Departamento de Ciencias Clínicas, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain
f Unidad de Cirugía Torácica and Trasplante Pulmonar de Donante en Asistolia, Hospital Clínico San Carlos, Madrid, Spain
This item has received
Article information
OBJECTIVE

To assess ischemia–reperfusion injury and early acute rejection of the lung subjected to ischemia for 10 hours.

MATERIAL AND METHODS

Fifteen of 30 Sprague–Dawley rats underwent transplantation of a left lung that had been subjected to ischemic times of 4 (n=5), 6 (n=5), or 10 hours (n=5). The cardiopulmonary block was removed from the donor, the left lung was dissected, and the transplant was carried out using the cuff technique. The cardiopulmonary block was extracted after 48 hours. We assessed postoperative progress, ischemia–reperfusion injury and acute rejection of the transplanted and contralateral lungs. Statistical probabilities were analyzed using the χ2 and Fisher exact tests.

RESULTS

Clinical course was not worse after an ischemic time of 10 hours (=.711). No significant differences were observed in histological markers of ischemia–reperfusion injury and acute rejection or in clinical course in relation to the different ischemic times; nor was clinical course related to the presence or severity of lesions or rejection. Similarly, acute rejection was unrelated to ischemia–reperfusion injury (>.05).

CONCLUSIONS

In this study, a prolonged ischemic time of 10 hours was not associated with ischemia–reperfusion injuries, with more severe acute rejection, or with a worse clinical course. Acute rejection was also unrelated to the presence or severity of ischemia–reperfusion injury.

Key words:
Lung transplantation
Ischemic time
Experimental surgery
OBJETIVO

Valorar la lesión de isquemia-reperfusión y el rechazo agudo precoz del pulmón sometido a un tiempo de isquemia de 10 h.

MATERIAL Y MÉTODOS

Se han utilizado 30 ratas Sprague-Dawley, en 15 de las cuales se realizó un trasplante pulmonar izquierdo con tiempos de isquemia de 4 h (n = 5), 6 h (n = 5) y 10 h (n = 5). Del donante se extrajo el bloque cardiopulmonar, se disecó el pulmón izquierdo y se efectuó el implante con la técnica de manguitos (cuffs). A las 48 h se extrajo el bloque cardiopulmonar. Se valoraron la evolución postoperatoria, la lesión de isquemia-reperfusión y el rechazo agudo del χ2 pulmón trasplantado y del contralateral. El análisis estadístico se realizó con el test de la χ2 y el test exacto de Fisher para el cálculo de probabilidades.

RESULTADOS

Los animales trasplantados con un tiempo de isquemia de 10 h no tuvieron peor evolución clínica (p = 0,711). No se observaron diferencias significativas entre los parámetros histológicos de lesión de isquemia-reperfusión y de rechazo agudo con los distintos tiempos de isquemia, ni en la evolución clínica por la presencia y gravedad de éstos. Tampoco se observó que el rechazo agudo se relacionara con la lesión de isquemia-reperfusión (p > 0,05).

CONCLUSIONES

En nuestro estudio, el tiempo de isquemia pulmonar prolongado de 10 h no se asocia ni a lesiones de isquemia-reperfusión y rechazo agudo más graves ni a una peor evolución clínica. El rechazo agudo no se relaciona con la presencia ni con la gravedad de la lesión de isquemiareperfusión.

Palabras clave:
Trasplante pulmonar
Tiempo de isquemia
Cirugía experimental
Full text is only aviable in PDF
REFERENCES
[1]
GI Snell, M Rabinov, A Griffiths.
Pulmonary allograft ischemic time: an important predictor of survival after lung transplantation.
J Heart Lung Transplant, 15 (1996), pp. 160-168
[2]
G Thabut, H Mal, J Cerrina, P Dartevelle, C Dromer, JF Velly.
Graft ischemic time and outcome of lung transplantation: a multicenter analysis.
Am J Respir Crit Care Med, 171 (2005), pp. 786-791
[3]
M Bund, H Struber, J Heine.
Effect of lung allograft ischemia duration on post reperfusion graft function and postoperative course.
Thorac Cardiovasc Surg, 46 (1998), pp. 93-96
[4]
T Ueno, GI Snell, TJ Williams.
Impact of graft ischemic time on outcomes after bilateral sequential single-lung transplantation.
Ann Thorac Surg, 67 (1999), pp. 1577-1582
[5]
RJ Novick, LE Benneth, DM Meyer.
Influence of graft ischemic time and donor age on survival after lung transplantation.
J Heart Lung Transplant, 18 (1999), pp. 425-431
[6]
AR Glanville, D Marshman, A Keogh.
Outcome in paired recipients of single lung transplants from the same donor.
J Heart Lung Transplant, 14 (1995), pp. 878-882
[7]
N Santana Rodríguez, L Martín Barrasa, A López García, P Rodríguez Suárez, M Ponce González, J Freixinet Gilart.
Trasplante pulmonar en ratas. Un modelo viable de estudio experimental.
Arch Bronconeumol, 40 (2004), pp. 438-442
[8]
M de Perrot, M Liu, T Waddell.
Ischemia-reperfusion-induced lung injury.
Am Resp J Crit Care Med, 167 (2003), pp. 490-511
[9]
S Sasaki, JD McCully, JD Palombo.
Lung preservation threshold in a compromised septic lung injury model.
Ann Thorac Surg, 60 (1995), pp. 958-963
[10]
C Serrick, A Giaid, A Reis.
Prolonged ischemia is associated with more pronounced rejection in the lung allograft.
Ann Thorac Surg, 63 (1997), pp. 202-208
[11]
KW Marck, J Prop, CR Wildevuur.
Lung transplantation in the rat: histopathology of left lung iso – and allografts.
J Heart Transplant, 4 (1985), pp. 263-266
[12]
T Shiraishi, T Mizuta, S DeMeester.
Effect of ischemic injury on subsequent rat lung allograft rejection.
Ann Thorac Surg, 60 (1995), pp. 947-951
[13]
AT Kawaguchi, T Mizuta, M Shirai.
Right lung transplantation followed by left pneumonectomy in the rat.
Eur J Cardiothorac Surg, 10 (1996), pp. 1011-1014
[14]
HC Paik, SC Hoffmann, TM Egan.
Pulmonary preservation studies: effects on endothelial function and pulmonary adenine nucleotides.
Transplantation, 75 (2003), pp. 439-444
[15]
A Watanabe, N Kawaharada, K Kusajima, S Komatsu, H Takahashi.
Contralateral lung injury associated with single-lung ischemiareperfusion injury.
Ann Thorac Surg, 62 (1996), pp. 1644-1649

This project was funded by the Spanish Health Research Fund (Fondo de Investigación Sanitaria Español, or FIS), the Canary Island Research Foundation (Fundación Canaria de Investigación y Salud, or FUNCIS, project 1104), the Canary Island Cancer Research Institute (Instituto Canario de Investigación del Cáncer, ICIC) and the Madrid MMA Foundation (Fundación MMA).

Copyright © 2007. Sociedad Española de Neumología y Cirugía Torácica (SEPAR)
Archivos de Bronconeumología
Article options
Tools

Are you a health professional able to prescribe or dispense drugs?