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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">La acidosis producida por aumento de CO<span class="elsevierStyleInf">2</span> disminuye la contractilidad y aumenta la fatigabilidad del m&#250;sculo estriado&#46; Se discute a qu&#233; niveles de CO<span class="elsevierStyleInf">2</span> y en qu&#233; modelos de experimentaci&#243;n tiene lugar este hecho&#46; Por ello&#44; se estudia en este trabajo el efecto de concentraciones crecientes de CO<span class="elsevierStyleInf">2</span> sobre la contractilidad y fatigabilidad en tiras de diafragma de rata in vitro&#46;</p><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Se estudiaron 6 tiras de diafragma de rata de bajo peso mantenidas en ba&#241;o de &#243;rganos a las que se aplicaron est&#237;mulos supram&#225;ximos de 1&#44; 10&#44; 50 y 100 Hz&#44; variando la concentraci&#243;n de CO<span class="elsevierStyleInf">2</span> de 5&#37; &#40;29 mmHg&#41;&#44; 10&#37; &#40;48&#44;3 mmHg&#41; y 20&#37; &#40;102 mmHg&#41;&#46; La fatigabilidad se estudi&#243; aplicando est&#237;mulos de 50 Hz&#44; 45 series por minuto durante 15 minutos&#46;</p><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Se obtuvieron los siguientes resultados&#58; <span class="elsevierStyleItalic">a&#41;</span> contractilidad&#46; La fuerza de contracci&#243;n fue menor con 20&#37; CO<span class="elsevierStyleInf">2</span> que con 5&#37; cuando se estimul&#243; con 1 Hz&#46; Para el resto de concentraciones y frecuencias no se encontraron diferencias significativas&#46; <span class="elsevierStyleItalic">b&#41;</span> Fatigabilidad&#46; La fuerza de contracci&#243;n fue menor a medida que se aumentaba la concentraci&#243;n de CO<span class="elsevierStyleInf">2</span>&#44; siendo significativas las diferencias con las 3 concentraciones&#46;</p><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Las conclusiones fueron&#58; <span class="elsevierStyleItalic">a&#41;</span> incrementos de CO<span class="elsevierStyleInf">2</span> de 5 a 20&#37; producen una significativa disminuci&#243;n en la fuerza de contracci&#243;n cuando se estimula a baja frecuencia&#44; y <span class="elsevierStyleItalic">b&#41;</span> aumentos de CO<span class="elsevierStyleInf">2</span> del 5 al 10 y 20&#37; hacen que el m&#250;sculo sea m&#225;s f&#225;cilmente fatigable&#46;</p></span>"
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        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Acidosis produced by an increase in CO<span class="elsevierStyleInf">2</span> concentration decreases contractility and increases the tendency to fatigue in striated muscle&#46; It is not clear a what levels of CO<span class="elsevierStyleInf">2</span> this effect takes place&#44; or in what experimental models it can be observed&#46; In this study we examine the effect of increasing concentrations of CO<span class="elsevierStyleInf">2</span> on contractility and fatigue in strips of rat diaphragm in vitro&#46;</p><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Six strips of diaphragm from a rat of low-weight were kept in organ bath and supramaximal stimuli of 1&#44; 10&#44; 50 and 100 Hz were applied while CO<span class="elsevierStyleInf">2</span> concentration was changed from 5&#37; &#40;29 mmHg&#41;&#44; to 10&#37; &#40;48&#46;3 mmHg&#41; to 20&#37; &#40;102 mmHg&#41;&#46; To study fatigue we applied 45 series of 50 Hz stimuli per minute for 15 minutes&#46;</p><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">We obtained these results&#58; <span class="elsevierStyleItalic">a&#41;</span> Contractility&#46; Forc&#233; of contraction was less with 20&#37; CO<span class="elsevierStyleInf">2</span> than with 5&#37; under a stimulus of 1 Hz&#46; No signif&#237;cant differences were found for other concentrations or frequencies&#46; <span class="elsevierStyleItalic">b&#41;</span> Fatigue&#46; Force of contraction decreases as CO<span class="elsevierStyleInf">2</span> concentration increased and signif&#237;cant differences were found for all 3 concentrations&#46;</p><p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">The conclusions were&#58; <span class="elsevierStyleItalic">a&#41;</span> an increase in CO<span class="elsevierStyleInf">2</span> from 5&#37; to 20&#37; produces a signif&#237;cant decrease in forc&#233; of contraction under conditions of low-frequency stimuli and <span class="elsevierStyleItalic">b&#41;</span> increases in CO<span class="elsevierStyleInf">2</span> concentration from 5&#37; to 10 to 20&#37; increase the muscle&#39;s susceptibility to fatigue&#46;</p></span>"
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Vol. 30. Issue 9.
Pages 445-448 (November 1994)
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Vol. 30. Issue 9.
Pages 445-448 (November 1994)
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Efecto del CO2 sobre el diafragma de rata in vitro
Impact of CO2 on the rat diaphragm in vitro
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4442
M.J. Barranco1, M.A. Ciscar, M. Ramón, G. Juan
Servicio de Neumología. Hospital General Universitario
J. Cortijo*
* Departamento de Farmacología. Facultad de Medicina. Valencia
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La acidosis producida por aumento de CO2 disminuye la contractilidad y aumenta la fatigabilidad del músculo estriado. Se discute a qué niveles de CO2 y en qué modelos de experimentación tiene lugar este hecho. Por ello, se estudia en este trabajo el efecto de concentraciones crecientes de CO2 sobre la contractilidad y fatigabilidad en tiras de diafragma de rata in vitro.

Se estudiaron 6 tiras de diafragma de rata de bajo peso mantenidas en baño de órganos a las que se aplicaron estímulos supramáximos de 1, 10, 50 y 100 Hz, variando la concentración de CO2 de 5% (29 mmHg), 10% (48,3 mmHg) y 20% (102 mmHg). La fatigabilidad se estudió aplicando estímulos de 50 Hz, 45 series por minuto durante 15 minutos.

Se obtuvieron los siguientes resultados: a) contractilidad. La fuerza de contracción fue menor con 20% CO2 que con 5% cuando se estimuló con 1 Hz. Para el resto de concentraciones y frecuencias no se encontraron diferencias significativas. b) Fatigabilidad. La fuerza de contracción fue menor a medida que se aumentaba la concentración de CO2, siendo significativas las diferencias con las 3 concentraciones.

Las conclusiones fueron: a) incrementos de CO2 de 5 a 20% producen una significativa disminución en la fuerza de contracción cuando se estimula a baja frecuencia, y b) aumentos de CO2 del 5 al 10 y 20% hacen que el músculo sea más fácilmente fatigable.

Palabras clave:
Contractilidad muscular
Fatigabilidad muscular
Hipercapnia

Acidosis produced by an increase in CO2 concentration decreases contractility and increases the tendency to fatigue in striated muscle. It is not clear a what levels of CO2 this effect takes place, or in what experimental models it can be observed. In this study we examine the effect of increasing concentrations of CO2 on contractility and fatigue in strips of rat diaphragm in vitro.

Six strips of diaphragm from a rat of low-weight were kept in organ bath and supramaximal stimuli of 1, 10, 50 and 100 Hz were applied while CO2 concentration was changed from 5% (29 mmHg), to 10% (48.3 mmHg) to 20% (102 mmHg). To study fatigue we applied 45 series of 50 Hz stimuli per minute for 15 minutes.

We obtained these results: a) Contractility. Forcé of contraction was less with 20% CO2 than with 5% under a stimulus of 1 Hz. No signifícant differences were found for other concentrations or frequencies. b) Fatigue. Force of contraction decreases as CO2 concentration increased and signifícant differences were found for all 3 concentrations.

The conclusions were: a) an increase in CO2 from 5% to 20% produces a signifícant decrease in forcé of contraction under conditions of low-frequency stimuli and b) increases in CO2 concentration from 5% to 10 to 20% increase the muscle's susceptibility to fatigue.

Key words:
Muscule
Contractility
Fatigue
Hypercapnia
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Copyright © 1994. Sociedad Española de Neumología y Cirugía Torácica
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