Experimental modelCompensatory Lung Growth in Autologous Lobar Implant: Experimental Study in Dogs
Section snippets
Materials and methods
We used 48 adult male dogs of undefined breed between (8 to 18 kg) which were provided by the UNESP Central Animal House and quarantined to assume that they were clinically healthy. The experiment was approved by the Ethics Committee on Animal Experimentation.
There were three experimental groups: control, G1 (n = 20), which were submitted to lung scintigraphy and then sacrificed for lung morphometry; lobectomy, G2 (n = 14) which underwent left cranial lobectomy and about 5 months later, lung
Results
The ischemia time varied from 57 to 85 minutes with maximum warm ischemia time of 4 minutes. G2 animals gained around 2.1 kg body mass between surgery and sacrifice; G3 animals gained only 0.7 kg. There was no significant difference in body mass between the three groups (P > .05).
G1, G2, and G3 left caudal lobe mean lung masses were 26.27 g, 35.20 g, and 43.19 g, respectively; significant differences were G1 < G2 < G3 (P < .001); RVs were 35.40 mL, 44.78 mL, and 48.64 mL respectively,
Left Caudal Lobe
Mean caudal lobe mass increased from G1 to G3, suggesting a higher mass compensatory growth in the reimplanted than in the remaining lobe of the lobectomized animals. In G3 we were able to measure the caudal lobe mass before reimplantation and therefore compare them at time of surgery and sacrifice. They were significantly higher at sacrifice even though they were drier due to bleeding. This mass in the reimplanted lobe was so large that it was similar to the whole left lung of the control
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Experimental Swine Lung Autotransplant Model to Study Lung Ischemia-Reperfusion Injury
2011, Archivos de BronconeumologiaCitation Excerpt :The majority of the research on pulmonary warm ischemia is done in small rodents by means of vascular occlusion techniques11,12 and less frequently include surgical procedures similar to those carried out in humans. Pulmonary autotransplantation in large mammals largely meets this objective, as reported in dogs,13–17 sheep18 and pigs,19 but few of these studies thoroughly analyze the inflammatory mediators during normothermic IR.20 In this present study, we analyzed the hemodynamic, blood gas and biochemical changes during pulmonary autotransplantation procedures in pigs.
Current and future treatment of fetal thoracic masses
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