Liver transplantationOutcomeHuman Leukocyte Antigen-G, a New Parameter in the Follow-up of Liver Transplantation
Section snippets
Patients and methods
Thirty-seven patients receiving primary liver transplants between 2004 and 2005 were enrolled in this prospective study. Serum samples were obtained just before and at 15 and 30 days after transplantation. The control group included 13 healthy blood donor volunteers. Peripheral mononuclear (PMNL) cells were isolated by density gradient centrifugation over Ficoll/Hypaque. We added IL-10 (50 ng/mL), cyclosporine (115 μg/mL), prednisolone (115 μg/mL), or cyclosporine + prednisolone (115 μg/mL +
Results
Among the control group, the mean value of sHLA-G level in serum was 7.2 ± 8.1 μg/mL and 61.7 ± 57.2 μg/mL in plasma. The flow cytometric analysis of cell cultures showed that within the first 24 hours IL-10 increased HLA-G expression on Treg cells the most (44304.0 ± 23143.0 for 54474.0 cells/mL), whereas prednisolone produced the same expression level within 48 hours (39091.0 ± 36608 for 44914 cells/mL) (Fig 1). On the other hand, the factor which increased soluble HLA-G serum levels at the
Discussion
The induction of allograft tolerance has traditionally been a basic aim of transplantation research. Multiple data from experimental models indicate that the outcome of transplantation (rejection versus acceptance/tolerance) depends on the balance between alloreactive cytopathic lymphocytes and immunoregulatory lymphocytes. Thus, most tolerance-inducing treatments seek to reduce the number of allo-aggressive lymphocytes and, at the same time, increase the population of regulatory lymphocytes,
References (18)
- et al.
Identification of HLA-G7 as a new splice variant of the HLA-G mRNA and expression of soluble HLA-G5, -G6, and -G7 transcripts in human transfected cells
Hum Immunol
(2000) - et al.
HLA-g suppresses proliferation of CD4(+) T lymphocytes
J Reprod Immunol
(2000) - et al.
Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver–kidney transplantation
J Hepatol
(2003) - et al.
The roles of CD25+CD4+ regulatory T cells in operational tolerance after living donor liver transplantation
Transplant Proc
(2005) - et al.
Decrease of CD4+CD25+ T cells in peripheral blood after liver transplantationassociation with immunosuppression
Transplant Proc
(2005) - et al.
Alternative splicing of HLA-G transcripts yields proteins with primary structures resembling both class I and class II antigens
Proc Natl Acad Sci USA
(1992) - et al.
HLA-G1 co-expression boots the HLA class I mediated NK lysis inhibition
Int Immunol
(2001) - et al.
HLAg2, -G3, and -G4 isoforms expressed as nonmature cell surface glycoproteins inhibit NK and antigen-specific CTL cytolysis
J Immunol
(2001) - et al.
Solule HLA-g protein secreted by allo-specific CD4+ T cells suppresses the alloproliferative responsea CD4+ T cell regulatory mechanism
Proct Natl Acad Sci USA
(2001)
Cited by (47)
Soluble HLA-G levels in heart transplant recipients: Dynamics and correlation with clinical outcomes
2023, Transplant ImmunologyCitation Excerpt :For example, a 14-bp insertion/deletion polymorphism located in exon 8 in the 3′ untranslated region of the HLA-G gene has found to be associated with the stability and splicing pattern of the mRNA, so resulting in a lower expression of HLA-G (35), (36), (37). Furthermore, environmental factors such as donor characteristics or the type of immunosuppressive regimen used (38), (39) may also play an important role in determining HLA-G expression of solid organ recipients. Sheshgiri et al. (40) reported that everolimus upregulates HLA-G expression, an effect which was not observed with other drugs such as mycophenolate mofetil or cyclosporine.
HLA-G and humanized mouse models as a novel therapeutic approach in transplantation
2020, Human ImmunologyNew Developments in HLA-G in Cardiac Transplantation
2016, Human ImmunologySoluble Human Leukocyte Antigen-G in the Bronchoalveolar Lavage of Lung Cancer Patients
2016, Archivos de BronconeumologiaBiology of the immunomodulatory molecule HLA-G in human liver diseases
2015, Journal of HepatologyCitation Excerpt :Numerous studies in the field of transplantation have demonstrated an association between high levels of HLA-G expression in biopsy specimens or high blood sHLA-G levels and higher rates of graft acceptance. Indeed, this association with a better post-transplantation outcome was first demonstrated for heart transplantation [70,71], and then for the transplantation of liver [39,61,72], kidney [73,74] and lung [75] grafts and for the transplantation of hematopoietic stem cells [68]. This beneficial effect may reflect a peripheral increase in the size of the CD3+CD4low and CD3+CD8low T-cell subpopulations associated with high levels of peripheral IL-10 production [76].
This work was supported by Baskent University project KA04/146.