TY - JOUR T1 - Expression of MicroRNA-133 Inhibits Epithelial–Mesenchymal Transition in Lung Cancer Cells by Directly Targeting FOXQ1 JO - Archivos de Bronconeumología T2 - AU - Xiao,Bo AU - Liu,Huazhen AU - Gu,Zeyun AU - Ji,Cheng SN - 15792129 M3 - 10.1016/j.arbr.2016.01.015 DO - 10.1016/j.arbr.2016.01.015 UR - https://archbronconeumol.org/en-expression-microrna-133-inhibits-epithelialmesenchymal-transition-articulo-S1579212916000185 AB - IntroductionMicroRNA (miR) was implicated in the tumorigenesis of many types of cancer, but no study was conducted on the exact role of miR-133 in lung cancer. MethodsWe have identified miR-133 as a putative regulator of FOXQ1 expression, and investigated the potential involvement of miR-133 in the migration and invasion of lung cancer cells, as well as the underlying molecular mechanism. ResultsMiR-133 directly targeted and down-regulated FOXQ1 expression, which in turn reduced TGF-β level. MiR-133 was down-regulated in lung cancer cell lines A549 and HCC827, and its re-expression significantly inhibited the migration and invasion of the lung cancer cells. Further investigation revealed that this inhibition was caused by reversing the epithelial–mesenchymal transition, evidenced by miR-133 induced elevation of epithelial marker E-cadherin, and reduction of mesenchymal marker Vimentin. ConclusionsOur study is the first to identify miR-133 as a biomarker for lung cancer. It functions to down-regulate FOXQ1, and inhibit epithelial–mesenchymal transition, which antagonizes lung cancer tumorigenesis. Therefore our data support the role of miR-133 as a potential molecular therapeutic tool in treating lung cancer. ER -