TY - JOUR T1 - Experimental Swine Lung Autotransplant Model to Study Lung Ischemia–Reperfusion Injury JO - Archivos de Bronconeumología T2 - AU - Simón Adiego,Carlos AU - González-Casaurrán,Guillermo AU - Azcárate Perea,Leire AU - Isea Viña,Jesús AU - Vara Ameigeiras,Elena AU - García Martín,Cruz AU - Garutti Martínez,Ignacio AU - Casanova Barea,Javier AU - Giráldez López,Ana AU - Martín Piñeiro,Beatriz AU - González-Aragoneses,Federico SN - 15792129 M3 - 10.1016/j.arbr.2011.02.002 DO - 10.1016/j.arbr.2011.02.002 UR - https://archbronconeumol.org/en-experimental-swine-lung-autotransplant-model-articulo-S1579212911000085 AB - IntroductionIschemia–reperfusion (IR) lung injury has been investigated extensively on clinical and experimental models of cold ischemia. However, relatively few studies examine the detailed biochemical changes occurring during normothermic (warm) IR. Animals and methodsSix large-white pigs underwent a lung autotransplant which entailed left pneumonectomy, ex situ cranial lobectomy, caudal lobe reimplantation and its reperfusion for 30min. Throughout the procedure, several parameters were measured in order to identify hemodynamic, gasometric and biochemical changes. Non-parametric statistical analyses were used to compare differences between periods. ResultsAfter ischemia, a significant increase (p<.05) in lipid peroxidation metabolites, proinflammatory cytokines and chemokines (TNF-α, IL-1β and MCP-1), neutrophil activation, inducible nitric oxide synthase activity and protein kinase MAPK p38 levels were observed in lung tissue. However, constitutive nitric oxide synthase activity in lung tissue and carbon monoxide plasma levels decreased. The same held true throughout the reperfusion period, when an increase in the constitutive heme-oxygenase activity was also shown. ConclusionsAn experimental model of normothermic lung IR injury is presented and detailed changes in hemodynamic, gasometric and biochemical parameters are shown. Both the model and the studied parameters may be clinically useful in future investigations testing new therapies to prevent normothermic IR induced lung injury. ER -